Home Binaries with Sfil restriction sites Binaries with Sfil restriction sites

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Revolution in Plant RNAi

In the section Vectors you will find new developements of double promoter vectors for plant RNAi.

Features:

  • Simplified cloning of RNAi constructs.
  • No size limit for RNAi inserts-silencing of several genes possible.
  • Cloning of cDNA libraries for phenotypescreening-please ask for assistance
Binaries with Sfil restriction sites
Agrobacterium Strains
Binaries with Sfil restriction sites
Agrobacterium Strain Chromosomal Background Marker Gene Ti PLasmid Marker Gene Opine
LBA4404 TiAch5 rif pAL4404 spec and strep Octopine
GV2260 C58 rif pGV2260 (pTiB6S3DT-DNA) carb Octopine
C58C1 C58 - Cured - Nopaline
GV3100 C58 - Cured - Nopaline
A136 C58 rif and nal Cured - Nopaline
GV3101 C58 rif Cured - Nopaline
GV3850 C58 rif pGV3850 (pTiC58Donc.genes) carb Nopaline
GV3101::pMP90 C58 rif pMP90 (pTiC58DT-DNA) gent Nopaline
GV3101::pMP90RK C58 rif pMP90RK (pTiC58DT-DNA) gent and kan Nopaline
EHA101 C58 rif pEHA101 (pTiBo542DT-DNA) kan Nopaline
EHA105 C58 rif pEHA105 (pTiBo542DT-DNA) - Succinamopine
AGL-1 C58, RecA rif, carb pTiBo542DT-DNA - Succinamopine
 
Binaries with dhfr as plant selection marker
Binaries with Sfil restriction sites
pLH5000 35S dhfr MCS-BA Basic binary for methotrexate selection genebank sequence image
p5AB 35S dhfr inverted MCS-BA Basic binary for methotrexate selection genebank sequence image
p5U ubi-maize dhfr MCS-BA Ubiquitin promotor from Z. mays genebank sequence image
Last Updated on Friday, 13 November 2009 18:17
 
Binaries with hpt as plant selection marker
Binaries with Sfil restriction sites
pLH6000 35S hpt
MCS-BA
Basic binary for hygromycine selection genebank sequence image
pLH6500 35S hpt
MCS-AB
Basic binary for hygromycine selection genebank sequence image
p6AB 35S hpt
inverted MCS-BA
Basic binary for hygromycine selection genebank sequence image
p6L 35S hpt
MCS-BA
Lox sites flanking selection marker genebank sequence image
p6i 35S hpt-intron
MCS-BA
Intron in plant selection marker genebank sequence image
p6-35S-OCS 35S hpt 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p6Nap 35S hpt Napin MCS-BA nos napin promotor expression cassette genebank sequence image










p6N NOS hpt
MCS-BA
For promotor analysis genebank sequence image
p6N-35S NOS hpt 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p6N-Gus NOS hpt
GUS nos For promotor analysis genebank sequence image
p6N-Gusi NOS hpt
GUS-intron nos For promotor analysis genebank sequence image










p6U ubi-maize hpt
MCS-BA
Ubiquitin promotor from Z. mays genebank sequence image
p65U ubi-maize hpt
MCS-AB
Ubiquitin promotor from Z. mays genebank sequence image
p6iU ubi-maize hpt-intron
MCS-BA
Ubiquitin promotor from Z. mays genebank sequence image










p6U10 UBQ10AT hpt
MCS-BA
Ubiquitin promotor from A. thaliana genebank sequence image
Last Updated on Friday, 13 November 2009 18:25
 
Binaries with bar as plant selection marker
Binaries with Sfil restriction sites
pLH7000 35S bar MCS-BA Basic binary for phoshinotricine selection genebank sequence image
pLH7000M 35S bar MCS-BA deleted SalI site in bar genebank sequence image
pLH7500 35S bar MCS-AB Basic binary for phoshinotricine selection genebank sequence image
p7AB 35S bar inverted MCS-BA Basic binary for phoshinotricine selection genebank sequence image
p7AB-P 35S bar inverted MCS-BA del.PstI site genebank sequence image
pLH75-P 35S bar MCS-AB del.PstI site genebank sequence image
pLH7-P 35S bar MCS-BA del.PstI site genebank sequence image
p7i-M 35S bar-intron MCS-BA deleted SalI site in bar genebank sequence image
p7i 35S bar-intron MCS-BA Intron in plant selection marker genebank sequence image
p7L 35S bar MCS-BA Lox sites flanking selection marker genebank sequence image
p7iL 35S bar-intron MCS-BA Lox sites flanking selection marker genebank sequence image
p7-35S-OCS 35S bar 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p7Nap 35S bar Napin MCS-BA nos napin promotor expression cassette genebank sequence image
p7UG-AB 35S bar ubi-maize GUS-intron nos For optimization of monocot transformation genebank sequence image
p7i-UG 35S bar-intron ubi-maize GUS nos For optimization of monocot transformation genebank sequence image
p7P-35Gus-iR 35S bar 35S Gus-intron-Gus ocs Vector for RNAi of Gus genebank sequence image
p75-GusRNAi 35S bar 35S Gus-spacer-Gus nos Vector for RNAi with spacer genebank sequence image
p7N NOS bar MCS-BA For promotor analysis genebank sequence image
p75N NOS bar MCS-AB For promotor analysis genebank sequence image
p7N-35S NOS bar 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p7N-Gus NOS bar GUS nos For promotor analysis genebank sequence image
p7N-Gusi NOS bar GUS-intron nos For promotor analysis genebank sequence image
p7U ubi-maize bar MCS-BA Ubiquitin promotor from Z. mays genebank sequence image
p75U ubi-maize bar MCS-AB Ubiquitin promotor from Z. mays genebank sequence image
p7iU ubi-maize bar-intron MCS-BA Ubiquitin promotor from Z. mays genebank sequence image
p7U10 UBQ10AT bar MCS-BA Ubiquitin promotor from A. thaliana genebank sequence image
pLH7-UBI-hel GUBB1HA bar MCS-BA Ubiquitin promotor from H. annuus genebank sequence image
p7U-GFP ubi-maize bar GFP GFP codonoptimized for monocots genebank sequence image
Last Updated on Friday, 11 June 2010 15:05
 
Binaries with npt as plant selection marker
Binaries with Sfil restriction sites
pLH9000 35S npt MCS-BA Basic binary for kanamycin selection genebank sequence image
pLH9500 35S npt MCS-AB Basic binary for kanamycin selection genebank sequence image
p9AB 35S npt inverted MCS-BA Basic binary for kanamycin selection genebank sequence image
p9L 35S npt MCS-BA Lox sites flanking selection marker genebank sequence image
p9i 35S npt-intron MCS-BA Intron in plant selection marker genebank sequence image
p9iL 35S npt-intron MCS-BA Lox sites flanking selection marker genebank sequence image
p9-35S-OCS 35S npt 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p9Nap 35S npt Napin MCS-BA nos napin promotor expression cassette genebank sequence image
p9-35s-Gus 35S npt 35S gus nos transformation optimization genebank sequence image
p9-35s-Gusi 35S npt 35S gus-intron nos transformation optimization genebank sequence image
p9-35s-EGFP 35S npt-intron 35S EGFP nos transformation optimization genebank sequence image
p9i-Ubi-Gusi 35S npt-intron Ubi gus-intron nos transformation optimization genebank sequence image
p9N NOS npt MCS-BA For promotor analysis genebank sequence image
p95N NOS npt MCS-AB For promotor analysis genebank sequence image
p95N-Gus NOS npt GUS nos For promotor analysis genebank sequence image
p9N-35S NOS npt 35S MCS-BA ocs 35S promotor expression cassette genebank sequence image
p9N-Gus NOS npt GUS nos For promotor analysis genebank sequence image
p9N-Gusi NOS npt GUS-intron nos For promotor analysis genebank sequence image
p9U ubi-maize npt MCS-BA Ubiquitin promotor from Z. mays genebank sequence image
p95U10 UBQ10AT npt MCS-AB Ubiquitin promotor from A. thaliana genebank sequence image
p9U10 UBQ10AT npt MCS-BA Ubiquitin promotor from A. thaliana genebank sequence image
pLH9-UBI-hel GUBB1HA npt MCS-BA Ubiquitin promotor from H. annuus genebank sequence image
Last Updated on Friday, 18 December 2009 15:20
 
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