LOCUS       B399p6oN-LH             9539 bp    DNA     circular SYN 27-JAN-2016
DEFINITION  Ligation of hpt-Xho-Mlu* into 715p7N-TE9*
ACCESSION   B399p6oN-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  715p7N-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8306
            Insert molecule:  hpt-Xho-Mlu*
               Fragment ends: XhoI and MluI
               Fragment size: 1233
            Primer for gene synthesis
            hptXho	CCCGCctcgagTCCTCCAtaactATGAAGAAACCGGAGC
            hptMlu	GCCATAATACTCGAACGCGTTCACTCC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..606
                     /gene="Pnos"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             625..1194
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    1195..1383
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1384..1839
                     /gene="hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1850..2140
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2316..2339
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2874..3571
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4120..7825
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8009..9019
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
      361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
      421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      601 ctgcacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa
      661 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa
      721 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct
      781 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg
      841 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc
      901 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc
      961 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc
     1021 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc
     1081 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg
     1141 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt
     1201 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     1261 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     1321 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     1381 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg
     1441 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg
     1501 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt
     1561 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc
     1621 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg
     1681 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc
     1741 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc
     1801 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca
     1861 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     1921 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     1981 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     2041 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     2101 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag
     2161 ctgatccact agaggccatg gcggccgcac tagtggatcc cccgggatgc atgaattcaa
     2221 gcttatcgat gtcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg
     2281 tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     2341 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     2401 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat
     2461 tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca
     2521 gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt
     2581 ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa
     2641 atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag
     2701 tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt
     2761 caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt
     2821 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga
     2881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3661 tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3721 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     3781 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3841 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     3901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3961 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4021 ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc
     4081 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     4141 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     4201 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     4261 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     4321 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     4381 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     4441 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     4501 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     4561 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     4621 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt
     4681 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4741 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     4801 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     4861 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     4921 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     4981 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     5041 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg
     5101 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5161 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5221 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5281 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5341 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5401 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     5461 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     5521 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     5581 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     5641 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5701 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     5761 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     5821 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     5881 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     5941 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6001 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6061 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6121 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6181 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6241 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     6301 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     6361 gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     6421 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     6481 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     6541 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     6601 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     6661 ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc
     6721 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     6781 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     6841 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     6901 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     6961 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     7021 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     7081 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     7141 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     7201 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     7261 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     7321 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     7381 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     7441 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     7501 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     7561 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     7621 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     7681 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     7741 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     7801 ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac
     7861 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
     7921 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     7981 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     8041 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     8101 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     8161 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     8221 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     8281 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     8341 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     8401 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     8461 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     8521 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     8581 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     8641 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     8701 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     8761 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     8821 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     8881 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     8941 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     9001 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg
     9061 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     9121 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     9181 tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc
     9241 ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga
     9301 tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca
     9361 acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct
     9421 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc
     9481 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc
//
