LOCUS A483p7i-TE9 9185 bp DNA circular SYN 31-MAY-2013 DEFINITION Ligation of 7i-Xho-Mlu* into 257p7-TE9* ACCESSION A483p7i-TE9 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9185) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 257p7-TE9* Fragment ends: MluI and XhoI Fragment size: 8426 Insert molecule: 7i-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 759 2-A483p7i-TE9 35RT3 CATTTCATTTGGAGAGGACCTC T35R2 CCCTTATCTGGGAACTACTC Sequencing primers 35S GCACAATCCCACTATCCTT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 741..974 /gene="bar" /product="Basta-Resistenz" /SECDrawAs="Gene" /SECStyleId=1 /SECName="bar" /SECDescr="Basta-Resistenz" misc_feature 975..1163 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" misc_feature 1164..1481 /gene="bar" /product="basta resistance" /SECDrawAs="Gene" /SECStyleId=1 /SECName="bar" /SECDescr="basta resistance" misc_feature 1496..1786 /gene="'E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1962..1985 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2520..3217 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3766..7471 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 7655..8665 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtggccacc atgggcccag aacgacgccc ggccgacatc cgccgtgcca 781 ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg 841 tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc 901 gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg 961 cgggcccctg gaaggtaagt ttctgcttct acctttgata tatatataat aattatcatt 1021 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag 1081 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat 1141 gaccaaaatt tgttgatgtg caggcacgca acgcctacga ctggacggcc gagtcgaccg 1201 tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc 1261 tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg 1321 acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg 1381 ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc 1441 cggtaccgcc ccgtccggtc ctgcccgtca ccgagatctg agatcacgcg ttcgagtatt 1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt 1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat 1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt 1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca 1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca 1801 tgcaagctga tccactagag gccatggcgg ccgcactagt ggatcccccg ggatgcatga 1861 attcaagctt atcgatgtcg acaggcctta agggccagat cttgggcccg gtacccgatc 1921 agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt tgacaggata tattggcggg 1981 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag 2041 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtgct 2101 tggcattccg tgcgataatg acttctgttc aaccacccaa acgtcggaaa gcctgacgac 2161 ggagcagcat tccaaaaaga tcccttggct cgtctgggtc ggctagaagg tcgagtgggc 2221 tgctgtggct tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga 2281 tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct 2341 ggccagtcat cacgcgccaa agttccgtca caggatgatc tggcgcgagt tgctggatct 2401 cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat 2461 cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccga 2521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 2581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 2641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 2701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 2761 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 2821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 2881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 2941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 3001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 3061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 3121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 3181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 3241 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 3301 attctgtgga taaccgatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 3361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 3421 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 3481 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct 3541 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3661 tcatcaccga aacgcgcgag gcaggggtac gtcgaggtcg atccaacccc tccgctgcta 3721 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa 3781 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg 3841 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa 3901 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac 3961 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac 4021 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga 4081 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat 4141 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga 4201 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga 4261 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cgcgaggcgt 4321 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat 4381 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac 4441 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg 4501 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg 4561 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc 4621 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc 4681 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct cgcggcctgg 4741 ccggcgagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt 4801 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa 4861 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca 4921 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt 4981 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac 5041 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc 5101 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga 5161 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca 5221 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag 5281 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg 5341 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc 5401 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc 5461 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa 5521 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag 5581 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca 5641 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat 5701 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa 5761 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca 5821 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct 5881 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag 5941 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 6001 gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 6061 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 6121 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 6181 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 6241 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 6301 cgtagaggtt tccgcagggc cggccggcat ggcgagtgtg tgggattacg acctggtact 6361 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 6421 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 6481 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 6541 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 6601 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 6661 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 6721 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 6781 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 6841 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 6901 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 6961 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 7021 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 7081 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 7141 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 7201 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 7261 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 7321 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 7381 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 7441 actcgaccgc cggcgcccac atcaaggcac cggtgggtat gcctgacgat gcgtggagac 7501 cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa 7561 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc 7621 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac 7681 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 7741 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg 7801 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac 7861 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt 7921 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc 7981 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt 8041 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc 8101 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat 8161 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga 8221 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac 8281 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc 8341 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc 8401 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag 8461 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct 8521 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga 8581 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga 8641 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc 8701 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat 8761 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga 8821 gatatatcat gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa 8881 catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact 8941 ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat ctcacggatc gactgcttct 9001 ctcgcaacgc catccgacgg atgatgttta aaagtcccat gtggatcact ccgttgcccc 9061 gtcgctcacc gtgttggggg gaaggtgcac atggctcagt tctcaatgga aattatctgc 9121 ctaaccggct cagttctgcg tagaaaccaa catgcaagct ccaccgggtg caaagcggca 9181 gcggc //