LOCUS       p9iTE9                  9433 bp    DNA     circular SYN 31-MAY-2013
DEFINITION  Ligation of 9i-Xho-Mlu* into 257p7-TE9*
ACCESSION   p9iTE9
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  257p7-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8426
            Insert molecule:  9i-Xho-Mlu*
               Fragment ends: XhoI and MluI
               Fragment size: 1007
            Primer for gene synthesis
            35RT	CTTCGCAAGACCCTTCCTCT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
            Sequencing primers	
            35S	GCACAATCCCACTATCCTT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             741..777
                     /gene="NptII"
                     /product="Kanamycin resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin resistance"
     misc_signal     746
                     /label=mut to C
                     /SECDrawAs="Label"
     misc_signal     749
                     /label=mut to C
                     /SECDrawAs="Label"
     misc_feature    778..966
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             967..1733
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin-Resistenz"
     misc_feature    1744..2034
                     /gene="'E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2210..2233
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2768..3465
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4014..7719
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7903..8913
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtggccacc atgggcgcct ggattgaaca agatggattg cacgcaggta
      781 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
      841 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
      901 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
      961 gtgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1441 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtt
     1741 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
     1801 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
     1861 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
     1921 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
     1981 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc
     2041 tgcaggcatg caagctgatc cactagaggc catggcggcc gcactagtgg atcccccggg
     2101 atgcatgaat tcaagcttat cgatgtcgac aggccttaag ggccagatct tgggcccggt
     2161 acccgatcag attgtcgttt cccgccttcg gtttaaacta tcagtgtttg acaggatata
     2221 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     2281 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     2341 ggagtgcttg gcattccgtg cgataatgac ttctgttcaa ccacccaaac gtcggaaagc
     2401 ctgacgacgg agcagcattc caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc
     2461 gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa
     2521 atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc
     2581 gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg
     2641 ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag
     2701 caagatatcg cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg
     2761 gacgccgaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3541 atcccctgat tctgtggata accgattacc gcctttgagt gagctgatac cgctcgccgc
     3601 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     3661 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     3721 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     3781 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3841 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3901 tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt cgaggtcgat ccaacccctc
     3961 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     4021 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     4081 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     4141 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     4201 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     4261 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     4321 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     4381 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     4441 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     4501 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg
     4561 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     4621 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     4681 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     4741 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     4801 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     4861 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     4921 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctcg
     4981 cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     5041 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     5101 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     5161 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     5221 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     5281 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     5341 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     5401 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     5461 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     5521 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     5581 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     5641 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     5701 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     5761 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     5821 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     5881 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     5941 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     6001 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     6061 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     6121 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     6181 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     6241 ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     6301 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     6361 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     6421 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     6481 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     6541 gacgggcacg tagaggtttc cgcagggccg gccggcatgg cgagtgtgtg ggattacgac
     6601 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     6661 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     6721 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     6781 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     6841 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     6901 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     6961 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     7021 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     7081 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     7141 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     7201 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     7261 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     7321 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     7381 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     7441 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     7501 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     7561 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     7621 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     7681 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg gtgggtatgc ctgacgatgc
     7741 gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg
     7801 ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     7861 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     7921 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     7981 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     8041 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     8101 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     8161 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8221 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8281 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8341 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8401 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8461 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8521 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8581 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8641 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8701 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8761 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8821 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     8881 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8941 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
     9001 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     9061 aattgggaga tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt
     9121 aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc ggctcgattg tacctgcgtt
     9181 caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt cggctgatct cacggatcga
     9241 ctgcttctct cgcaacgcca tccgacggat gatgtttaaa agtcccatgt ggatcactcc
     9301 gttgccccgt cgctcaccgt gttgggggga aggtgcacat ggctcagttc tcaatggaaa
     9361 ttatctgcct aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
     9421 aagcggcagc ggc
//
