LOCUS       p6i-UbiHa              10201 bp    DNA     circular SYN 31-MAY-2013
DEFINITION  Ligation of pUbiH-Nos* into p6i*
ACCESSION   p6i-UbiHa
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p6i*
               Fragment ends: SfiI
               Fragment size: 9526
            Insert molecule:  pUbiH-Nos*
               Fragment ends: SfiI
               Fragment size: 675
            GELR
            Sequencing primers	
            NosU	CAAGACCGGCAACAGGATTC
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S"
                     /SECDescr="35S-Promotor"
     misc_feature    452..869
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hpt"
                     /SECDescr="Hygromycin"
     misc_feature    870..1058
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron STLS1"
     misc_feature    1059..1666
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hpt"
                     /SECDescr="Hygromycin"
     misc_feature    1691..1892
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="T35S"
                     /SECDescr="Terminator 35S"
     misc_feature    1941..2276
                     /gene="Ubi-Prom"
                     /product="Ubi Promotor GUBB1HA"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2337..2590
                     /gene="Tnos"
                     /product="NOS terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2676..2699
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="BR"
                     /SECDescr="right border"
     misc_feature    3234..3931
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1 ORI"
     misc_feature    4480..8185
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pVS1 ORI"
                     /SECDescr="pVS1 ORI"
     CDS             8369..9379
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Sm/Sp"
                     /SECDescr="Strep/Spec resistence"
     misc_feature    9900..9923
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="BL"
                     /SECDescr="left border"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag ggccccgggg ggcaataaga tatgaaaaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg taagtttctg cttctacctt tgatatatat
      901 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa
      961 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata
     1021 acttttctaa tatatgacca aaatttgttg atgtgcaggt atcggtcaat acactacatg
     1081 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     1141 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     1201 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     1261 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     1321 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     1381 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     1441 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     1501 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     1561 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     1621 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc
     1681 ggagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag
     1741 aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg
     1801 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
     1861 aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcaagc tgatccacta
     1921 gaggccatgg cggccgcgac aaaacttaag aaagtcacca aaaagaaagt caaagacggc
     1981 aacggcaacc cagaagctac gttctctttc tagattctat gccctaccta ccgcgtggtg
     2041 cacaacaact tgacccccat cattgactct ccaccgtaca cgtgtcacga taccatttgt
     2101 tggtctccta ccacttacgt aacattaata ttccatccgt gaccgcctct tcttcatcat
     2161 cctataaatt caccttctta tctcttccgc tttcattcgt tcatcatcat cacaattaac
     2221 aacaatccaa agcaatcttc tctcatattc ttatctcttc ctgtttcgct tcgtctacta
     2281 gtggatcccc cgggctgcag gaattcaagc ttacgcgtgt cgactcgaat ttccccgatc
     2341 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     2401 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     2461 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     2521 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     2581 tactagatcg ctcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg
     2641 tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     2701 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     2761 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat
     2821 tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca
     2881 gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt
     2941 ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa
     3001 atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag
     3061 tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt
     3121 caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt
     3181 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga
     3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3481 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3901 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3961 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4021 tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4081 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     4141 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     4201 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     4261 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4321 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4381 ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc
     4441 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     4501 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     4561 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     4621 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     4681 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     4741 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     4801 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     4861 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     4921 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     4981 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt
     5041 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     5101 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     5161 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     5221 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     5281 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     5341 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     5401 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg
     5461 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5521 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5581 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5641 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5701 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5761 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     5821 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     5881 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     5941 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     6001 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     6061 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     6121 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     6181 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     6241 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     6301 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6361 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6421 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6481 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6541 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6601 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     6661 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     6721 gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     6781 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     6841 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     6901 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     6961 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     7021 ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc
     7081 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     7141 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     7201 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     7261 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     7321 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     7381 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     7441 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     7501 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     7561 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     7621 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     7681 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     7741 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     7801 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     7861 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     7921 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     7981 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     8041 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     8101 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     8161 ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac
     8221 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
     8281 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     8341 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     8401 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     8461 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     8521 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     8581 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     8641 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     8701 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     8761 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     8821 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     8881 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     8941 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     9001 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     9061 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     9121 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     9181 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     9241 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     9301 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     9361 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg
     9421 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     9481 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     9541 tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc
     9601 ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga
     9661 tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca
     9721 acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct
     9781 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc
     9841 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg
     9901 gcaggatata ttcaattgta aatggcttca tgtccgggaa atctacatgg atcagcaatg
     9961 agtatgatgg tcaatatgga gaaaaagaaa gagtaattac caattttttt tcaattcaaa
    10021 aatgtagatg tccgcagcgt tattataaaa tgaaagtaca ttttgataaa acgacaaatt
    10081 acgatccgtc gtatttatag gcgaaagcaa taaacaaatt attctaattc ggaaatcttt
    10141 atttcgacgt gtctacattc acgtccaaat gggggcttag atgagaaact tcacgatcgg
    10201 c
//
