LOCUS       p7i-UbiHa               9711 bp    DNA     circular SYN 31-MAY-2013
DEFINITION  Ligation of pUbiH-Nos* into p7iM*
ACCESSION   p7i-UbiHa
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7iM*
               Fragment ends: SfiI
               Fragment size: 9036
            Insert molecule:  pUbiH-Nos*
               Fragment ends: SfiI
               Fragment size: 675
            Sequencing primers	
            NosU	CAAGACCGGCAACAGGATTC
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             439..672
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             673..861
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             862..1179
                     /gene="bar"
                     /product="basta resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1201..1402
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1451..1786
                     /gene="Ubi"
                     /product="Promotor Helianthus anuus GUBB1HA"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1847..2100
                     /gene="Tnos"
                     /product="NOS-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2186..2209
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2744..3441
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3990..7695
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7879..8889
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    9410..9433
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc
      481 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
      541 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
      601 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
      661 ggcccctgga aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
      721 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
      781 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
      841 ccaaaatttg ttgatgtgca ggcacgcaac gcctacgact ggacggccga gtccaccgtg
      901 tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg
      961 aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac
     1021 ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc
     1081 ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg
     1141 gtaccgcccc gtccggtcct gcccgtcacc gagatctgag atcacgcgtt ctagtccgca
     1201 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
     1261 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
     1321 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     1381 ttcctaaaac caaaatccag tgacctgcag gcatgcaagc tgatccacta gaggccatgg
     1441 cggccgcgac aaaacttaag aaagtcacca aaaagaaagt caaagacggc aacggcaacc
     1501 cagaagctac gttctctttc tagattctat gccctaccta ccgcgtggtg cacaacaact
     1561 tgacccccat cattgactct ccaccgtaca cgtgtcacga taccatttgt tggtctccta
     1621 ccacttacgt aacattaata ttccatccgt gaccgcctct tcttcatcat cctataaatt
     1681 caccttctta tctcttccgc tttcattcgt tcatcatcat cacaattaac aacaatccaa
     1741 agcaatcttc tctcatattc ttatctcttc ctgtttcgct tcgtctacta gtggatcccc
     1801 cgggctgcag gaattcaagc ttacgcgtgt cgactcgaat ttccccgatc gttcaaacat
     1861 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     1921 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     1981 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2041 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     2101 ctcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg
     2161 ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     2221 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     2281 atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat
     2341 aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa
     2401 aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc
     2461 ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct
     2521 gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg
     2581 ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt
     2641 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
     2701 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa
     2761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3541 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3601 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     3661 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3721 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     3781 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3841 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3901 cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc
     3961 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     4021 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     4081 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     4141 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     4201 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     4261 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     4321 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     4381 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     4441 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     4501 gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc
     4561 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     4621 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     4681 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     4741 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     4801 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     4861 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     4921 atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg
     4981 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     5041 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     5101 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     5161 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     5221 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     5281 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     5341 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     5401 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     5461 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     5521 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     5581 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     5641 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     5701 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     5761 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     5821 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     5881 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     5941 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     6001 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     6061 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     6121 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     6181 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg
     6241 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     6301 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     6361 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     6421 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     6481 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     6541 gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     6601 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     6661 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     6721 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     6781 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     6841 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     6901 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     6961 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     7021 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     7081 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     7141 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     7201 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     7261 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     7321 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     7381 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     7441 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     7501 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     7561 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     7621 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     7681 ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
     7741 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
     7801 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     7861 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     7921 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     7981 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     8041 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     8101 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     8161 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     8221 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     8281 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     8341 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     8401 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     8461 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     8521 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     8581 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     8641 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     8701 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     8761 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     8821 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     8881 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     8941 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     9001 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     9061 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc
     9121 gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg
     9181 cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg
     9241 acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg
     9301 gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc
     9361 tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg gcaggatata
     9421 ttcaattgta aatggcttca tgtccgggaa atctacatgg atcagcaatg agtatgatgg
     9481 tcaatatgga gaaaaagaaa gagtaattac caattttttt tcaattcaaa aatgtagatg
     9541 tccgcagcgt tattataaaa tgaaagtaca ttttgataaa acgacaaatt acgatccgtc
     9601 gtatttatag gcgaaagcaa taaacaaatt attctaattc ggaaatcttt atttcgacgt
     9661 gtctacattc acgtccaaat gggggcttag atgagaaact tcacgatcgg c
//
