LOCUS       p9U10-35s              10847 bp    DNA     circular SYN 31-MAY-2013
DEFINITION  Ligation of p35-OCS-BM* into p9U10*
ACCESSION   p9U10-35s
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p9U10*
               Fragment ends: SfiI
               Fragment size: 10155
            Insert molecule:  p35-OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1786
                     /gene="UBQ10"
                     /product="Promotor Ubi A.thaliana"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1415..1480
                     /product="Ubi cds 5`"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1481..1784
                     /gene="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1806..2609
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2622..2823
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2875..3297
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S-Pro"
                     /SECDescr="Promotor-Region"
     misc_feature    3344..3539
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3624..3647
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4182..4879
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5428..9133
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9317..10327
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagtgc tttccttaca ttctgagcct ctttccttct aatccactca tctgcatctt
      361 cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc accagctcgt
      421 ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca ttcttcagat
      481 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
      541 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
      601 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
      661 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
      721 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
      781 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
      841 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
      901 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
      961 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     1021 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     1081 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
     1141 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     1201 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     1261 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     1321 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
     1381 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     1441 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     1501 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     1561 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc
     1621 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     1681 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     1741 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagatacta gctcgagtgg
     1801 ccaccatggg agcttggatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1861 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     1921 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     1981 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     2041 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     2101 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     2161 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2221 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     2281 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     2341 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     2401 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     2461 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     2521 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     2581 tctatcgcct tcttgacgag ttcttctgag cgggacccgc aaaaatcacc agtctctctc
     2641 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     2701 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     2761 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     2821 gtgacctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac tagatcaaca
     2881 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     2941 agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     3001 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat
     3061 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca
     3121 aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt
     3181 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     3241 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacactag
     3301 ttggatcccc cgggctgcag gaattcaagc ttacgcgtgt cgaccctgct ttaatgagat
     3361 atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa
     3421 aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata
     3481 tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtcct
     3541 cgacaggcct taagggccag atcttgggcc cggtacccga tcagattgtc gtttcccgcc
     3601 ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     3661 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     3721 ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc cgtgcgataa
     3781 tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc attccaaaaa
     3841 gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct
     3901 caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt
     3961 cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc
     4021 aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca atccgggtct
     4081 gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct
     4141 tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc taggtgaaga
     4201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgat
     4981 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5041 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5101 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5161 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     5221 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5281 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5341 aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag tcggcttctg
     5401 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     5461 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     5521 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     5581 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     5641 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     5701 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     5761 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     5821 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     5881 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     5941 ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc
     6001 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     6061 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     6121 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     6181 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     6241 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     6301 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     6361 gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag cttggccgct
     6421 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     6481 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     6541 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     6601 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     6661 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     6721 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     6781 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     6841 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     6901 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     6961 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     7021 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     7081 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     7141 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     7201 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     7261 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     7321 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     7381 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     7441 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     7501 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     7561 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     7621 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca
     7681 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     7741 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     7801 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     7861 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     7921 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     7981 gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     8041 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     8101 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     8161 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     8221 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     8281 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     8341 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     8401 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     8461 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     8521 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     8581 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     8641 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     8701 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     8761 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     8821 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     8881 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     8941 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     9001 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     9061 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     9121 acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
     9181 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     9241 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     9301 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     9361 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     9421 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     9481 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     9541 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     9601 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     9661 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     9721 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     9781 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     9841 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     9901 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     9961 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
    10021 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
    10081 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
    10141 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
    10201 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
    10261 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
    10321 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
    10381 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
    10441 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct
    10501 ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt gcttggtcgt
    10561 tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg
    10621 cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac gccatccgac
    10681 ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg
    10741 gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg
    10801 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc
//
