LOCUS       p9i-UbiATm             11019 bp    DNA     circular SYN 31-MAY-2013
DEFINITION  Ligation of 204pUbiAtmu-ocs* into p9i*
ACCESSION   p9i-UbiATm
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p9i*
               Fragment ends: SfiI
               Fragment size: 9279
            Insert molecule:  204pUbiAtmu-ocs*
               Fragment ends: SfiI
               Fragment size: 1740
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="BL"
                     /SECDescr="Border-Left"
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S"
                     /SECDescr="35S-Promotor"
     CDS             741..777
                     /gene="NptII"
                     /product="Kanamycin resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin resistance"
     misc_feature    778..966
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             967..1733
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin-Resistenz"
     misc_feature    1746..1947
                     /gene="T-35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="T-35S"
                     /SECDescr="Terminator 35S"
     misc_feature    1993..3468
                     /gene="Ubq10At"
                     /product="Promoter-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3097..3160
                     /product="Ubi cds 5`"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3163..3466
                     /product="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3516..3711
                     /gene="ocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ocs"
                     /SECDescr="Terminator"
     misc_feature    3796..3819
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="BR"
                     /SECDescr="Border-right"
     misc_feature    4354..5051
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1 ORI"
     misc_feature    5600..9305
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pVS1 ORI"
                     /SECDescr="pVS1 ORI"
     CDS             9489..10499
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Sm/Sp"
                     /SECDescr="Strep/Spec resistence"
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtggccacc atgggagctt ggattgaaca agatggattg cacgcaggta
      781 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
      841 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
      901 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
      961 gtgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1441 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     1741 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     1801 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     1861 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     1921 tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg caagctgatc cactagaggc
     1981 catggcggcc gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
     2041 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
     2101 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
     2161 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
     2221 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
     2281 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
     2341 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
     2401 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
     2461 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaattaaa
     2521 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
     2581 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
     2641 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
     2701 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
     2761 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaga
     2821 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
     2881 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
     2941 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
     3001 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
     3061 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     3121 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     3181 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     3241 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     3301 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     3361 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     3421 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacaggtac tagtggatcc
     3481 cccgggctgc aggaattcaa gcttacgcgt gtcgaccctg ctttaatgag atatgcgaga
     3541 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     3601 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     3661 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgtc ctcgacaggc
     3721 cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt
     3781 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     3841 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     3901 gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct
     3961 gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt
     4021 ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg
     4081 tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc
     4141 gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc
     4201 gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg
     4261 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg
     4321 cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt
     4381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct
     5161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5221 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5281 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     5341 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     5401 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5461 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     5521 gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     5581 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     5641 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     5701 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     5761 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     5821 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     5881 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     5941 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     6001 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     6061 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     6121 ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca
     6181 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     6241 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     6301 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     6361 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     6421 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     6481 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     6541 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac
     6601 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     6661 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     6721 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     6781 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     6841 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     6901 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     6961 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     7021 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     7081 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     7141 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     7201 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     7261 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     7321 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     7381 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     7441 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     7501 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     7561 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     7621 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     7681 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     7741 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     7801 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc
     7861 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     7921 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     7981 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     8041 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     8101 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     8161 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     8221 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     8281 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     8341 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     8401 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     8461 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     8521 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     8581 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     8641 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     8701 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     8761 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     8821 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     8881 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     8941 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     9001 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     9061 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     9121 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     9181 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     9241 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     9301 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
     9361 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     9421 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     9481 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     9541 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     9601 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     9661 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     9721 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     9781 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     9841 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     9901 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     9961 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
    10021 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
    10081 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
    10141 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
    10201 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
    10261 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
    10321 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
    10381 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
    10441 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
    10501 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
    10561 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
    10621 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt
    10681 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg
    10741 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg
    10801 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg
    10861 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt
    10921 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa
    10981 ccaacatgca agctccaccg ggtgcaaagc ggcagcggc
//
