LOCUS       p6iK                    9583 bp    DNA     circular SYN 13-JUN-2013
DEFINITION  Ligation of p6i* into 840p7iUzK*
ACCESSION   p6iK
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  840p7iUzK*
               Fragment ends: SbfI and XbaI
               Fragment size: 7685
            Insert molecule:  p6i*
               Fragment ends: XbaI and SbfI
               Fragment size: 1898
            Sequencing primers
            LHX	CGTGTCTACATTCACGTCC
            LH1.2	CGAAGGCGGGAAACGACAAT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    350..765
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S"
                     /SECDescr="35S-Promotor"
     misc_feature    795..1212
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hpt"
                     /SECDescr="Hygromycin"
     misc_feature    1213..1401
                     /gene="STLS1"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="STLS1"
                     /SECDescr="Intron STLS1"
     misc_feature    1402..2009
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hpt"
                     /SECDescr="Hygromycin"
     misc_feature    2034..2235
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="T35S"
                     /SECDescr="Terminator 35S"
     misc_feature    2408..2431
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2966..3663
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4212..7917
                     /gene="pVS1 ORI'"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8248..9063
                     /gene="'Kan"
                     /product="Kan"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagtag tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagaa catggtggag
      361 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccagagggct
      421 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat
      541 tgcgataaag gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga
      601 cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggacctc gagggccccg
      781 gggggcaata agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
      841 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
      901 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
      961 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     1021 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     1081 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     1141 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     1201 tcggaccgca aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1261 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1321 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1381 ccaaaatttg ttgatgtgca ggtatcggtc aatacactac atggcgtgat ttcatatgcg
     1441 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     1501 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     1561 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     1621 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     1681 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     1741 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     1801 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     1861 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     1921 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     1981 ccagcactcg tccgagggca aaggaataga gtagatgccg accggagtcc gcaaaaatca
     2041 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     2101 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     2161 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2221 aaccaaaatc cagtgacctg caggcatgca agctgatcca ctagaggcca tggcggccgc
     2281 actagtggat cccccgggat gcatgaattc aagcttatcg atgtcgacag gccttaaggg
     2341 ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc
     2401 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     2461 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     2521 accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc
     2581 acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc
     2641 tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac
     2701 gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg
     2761 cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg
     2821 atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac
     2881 gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc
     2941 gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct
     3001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3721 catgttcttt cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga
     3781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3901 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3961 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     4021 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4081 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg
     4141 aggtcgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     4201 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     4261 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     4321 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     4381 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     4441 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     4501 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     4561 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     4621 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     4681 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     4741 cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc gccctaccct caccccggca
     4801 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     4861 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     4921 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     4981 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     5041 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     5101 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     5161 tgtctgatgc caagctcgcg gcctggccgg cgagcttggc cgctgaagaa accgagcgcc
     5221 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     5281 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     5341 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     5401 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     5461 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     5521 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     5581 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     5641 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     5701 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     5761 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     5821 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     5881 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     5941 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     6001 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     6061 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     6121 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     6181 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     6241 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     6301 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     6361 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     6421 gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa
     6481 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     6541 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     6601 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     6661 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     6721 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcg
     6781 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     6841 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     6901 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     6961 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     7021 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     7081 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     7141 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     7201 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     7261 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     7321 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     7381 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     7441 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     7501 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     7561 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     7621 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     7681 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     7741 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     7801 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     7861 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccggt
     7921 tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca
     7981 tccagccaga aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg
     8041 gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc
     8101 tgatccttca actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat
     8161 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     8221 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
     8281 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     8341 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     8401 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     8461 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     8521 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
     8581 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     8641 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     8701 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     8761 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
     8821 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     8881 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     8941 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     9001 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     9061 taatgtctaa caattcgttc aagccgacgc cgcttcgtgg cgcggcttaa ctcaagcgtt
     9121 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     9181 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct
     9241 ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg
     9301 cgtgaacgtc ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg
     9361 cccggtgcgt cggctgatct cacggatcga ctgcttctct cgcaacgcca tccgacggat
     9421 gatgtttaaa agtcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga
     9481 aggtgcacat ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta
     9541 gaaaccaaca tgcaagctcc accgggtgca aagcggcagc ggc
//
