LOCUS p6iUAtK 10635 bp DNA circular SYN 13-JUN-2013 DEFINITION Ligation of Ubi-HPTi-Nhe-Sbf* into A209p6iK* ACCESSION p6iUAtK KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10635) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: A209p6iK* Fragment ends: SbfI and XbaI Fragment size: 7685 Insert molecule: Ubi-HPTi-Nhe-Sbf* Fragment ends: NheI and SbfI Fragment size: 2950 Primer for gene synthesis UbiNhe CCCTTgctagccctagGCTTTCCTTACATTCTGA UbiR cattgttagtcgacGGTACCTGTTAATCAGAA HptF ctgattaacaggtaccgtcgactaacaATGAAAAAGCCTGAACTC T35Sbf CTCTAGTGGATCAGCTTGCATGCCT Sequencing primers LHX CGTGTCTACATTCACGTCC HPT-R GACAGACGTCGCGGTGAGTT Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT LH1.2 CGAAGGCGGGAAACGACAAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 357..1832 /gene="Ubq10At" /product="Promoter-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1527..1830 /gene="int" /product="íntron ubi" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1847..2264 /gene="'hpt" /product="Hygromycin" /SECDrawAs="Gene" /SECStyleId=1 /SECName="'hpt" /SECDescr="Hygromycin" misc_feature 2265..2453 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" misc_feature 2454..3061 /gene="hpt" /product="Hygromycin" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hpt" /SECDescr="Hygromycin" misc_feature 3086..3287 /gene="T35S" /product="Terminator 35S" /SECDrawAs="Region" /SECStyleId=1 /SECName="T35S" /SECDescr="Terminator 35S" misc_feature 3460..3483 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4018..4715 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5264..8969 /gene="pVS1 ORI'" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 9300..10115 /gene="'Kan" /product="Kan" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagtag tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagcc ctaggctttc 361 cttacattct gagcctcttt ccttctaatc cactcatctg catcttcttg tgtccttact 421 aatacctcat tggttccaaa ttccctccct ttaagcacca gctcgtttct gttcttccac 481 agcctcccaa gtatccaagg gactaaagcc tccacattct tcagatcagg atattcttgt 541 ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc 601 ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta 661 ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc 721 ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca 781 cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa 841 atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc 901 acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc 961 acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat 1021 tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt 1081 ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa 1141 cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac 1201 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca 1261 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt 1321 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct 1381 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac 1441 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc 1501 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct 1561 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa 1621 tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta 1681 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc 1741 ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg 1801 tcgattaatc tgagtttttc tgattaacag gtaccgtcga ctaacaatga aaaagcctga 1861 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct 1921 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 1981 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg 2041 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga 2101 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 2161 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc 2221 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggtaagt ttctgcttct 2281 acctttgata tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt 2341 tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt 2401 atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtatcgg 2461 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg 2521 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat 2581 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa 2641 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt 2701 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat 2761 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct 2821 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa 2881 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg 2941 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt 3001 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata 3061 gagtagatgc cgaccggagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc 3121 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat 3181 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa 3241 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg 3301 caagctgatc cactagaggc catggcggcc gcactagtgg atcccccggg atgcatgaat 3361 tcaagcttat cgatgtcgac aggccttaag ggccagatct tgggcccggt acccgatcag 3421 attgtcgttt cccgccttcg gtttaaacta tcagtgtttg acaggatata ttggcgggta 3481 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt 3541 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtgcttg 3601 gcattccgtg cgataatgac ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg 3661 agcagcattc caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg 3721 ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc 3781 gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg 3841 ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg 3901 ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatatcg 3961 cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg gacgccgaaa 4021 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4081 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4141 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4201 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4261 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4321 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4381 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4441 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4501 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4561 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4621 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4681 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4741 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4801 tctgtggata accgattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 4861 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 4921 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 4981 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 5041 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 5101 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 5161 atcaccgaaa cgcgcgaggc aggggtacgt cgaggtcgat ccaacccctc cgctgctata 5221 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt 5281 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt 5341 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga 5401 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca 5461 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg 5521 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg 5581 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg 5641 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca 5701 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc 5761 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg cgaggcgtga 5821 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg 5881 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc 5941 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg 6001 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc 6061 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga 6121 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc 6181 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctcg cggcctggcc 6241 ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga 6301 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat 6361 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag 6421 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta 6481 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg 6541 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg 6601 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc 6661 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc 6721 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg 6781 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg 6841 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca 6901 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc 6961 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag 7021 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca 7081 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt 7141 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta 7201 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg 7261 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc 7321 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg 7381 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg 7441 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga 7501 agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg 7561 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg 7621 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga 7681 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc 7741 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg 7801 tagaggtttc cgcagggccg gccggcatgg cgagtgtgtg ggattacgac ctggtactga 7861 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc 7921 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg 7981 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg 8041 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag 8101 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga 8161 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga 8221 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg 8281 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca 8341 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa 8401 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca 8461 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga 8521 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata 8581 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc 8641 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag 8701 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct 8761 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt 8821 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa 8881 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac 8941 tcgaccgccg gcgcccacat caaggcaccg gttgaggtct gcctcgtgaa gaaggtgttg 9001 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt 9061 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg 9121 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat 9181 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa 9241 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 9301 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg 9361 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 9421 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 9481 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 9541 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 9601 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 9661 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 9721 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 9781 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 9841 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 9901 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 9961 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 10021 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 10081 tgcagtttca tttgatgctc gatgagtttt tctaatgtct aacaattcgt tcaagccgac 10141 gccgcttcgt ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca 10201 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca 10261 caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa 10321 gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat tgtacctgcg 10381 ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat ctcacggatc 10441 gactgcttct ctcgcaacgc catccgacgg atgatgttta aaagtcccat gtggatcact 10501 ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt tctcaatgga 10561 aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct ccaccgggtg 10621 caaagcggca gcggc //