LOCUS       p6MoI-asd              10034 bp    DNA     circular SYN 12-JUN-2013
DEFINITION  Ligation of asd-koz-mlu-xho* into 611p6moD35*
ACCESSION   p6MoI-asd
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  611p6moD35*
               Fragment ends: MluI and XhoI
               Fragment size: 8798
            Insert molecule:  asd-koz-mlu-xho*
               Fragment ends: XhoI and MluI
               Fragment size: 1236
            Sequencing Primer:
            T35Sbf
            35RT
FEATURES             Location/Qualifiers
     misc_feature    67..814
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    815..850
                     /product="5´UTR"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             872..1441
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    1442..1630
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1631..2086
                     /gene="'hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2097..2387
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2563..2586
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3121..3818
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4367..8072
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8256..9266
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    9787..9810
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga
       61 tcgggccaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      121 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      181 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
      241 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
      301 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
      361 aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct
      421 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
      481 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
      541 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
      601 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      661 gcatcgtgga aaaagaagac cttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      721 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
      781 tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa
      841 atctatctct ctcgagggat cctccacaac catgaagaaa ccggagctga ccgccacttc
      901 cgtcgaaaag ttcctcatcg agaagtttga tagcgtgtct gaccttatgc agttgtcgga
      961 gggcgaagag tcccgcgcgt tcagctttga tgttggcggg cgcgggtacg tcctccgcgt
     1021 gaactcttgc gctgacggct tctataaaga tcggtacgtt tacaggcatt tcgcctcggc
     1081 ggccctgcct attcccgaag tcctcgacat cggcgagttt tccgagagcc ttacgtattg
     1141 tatttcccgc cgggctcaag gggtgaccct ccaggatctg ccggaaactg agttgcctgc
     1201 ggttctccag cccgtcgccg aggctatgga cgccatcgcc gcggcggatc tttctcaaac
     1261 gtcgggcttc gggccgttcg gccctcaggg gattggccag tacaccactt ggagggactt
     1321 tatctgcgct atcgccgatc cgcacgtgta tcattggcaa accgttatgg acgatacggt
     1381 ctccgcgagc gtggcccagg ctctggacga actcatgttg tgggccgagg attgccccga
     1441 ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat
     1501 ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct
     1561 gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt
     1621 tgatgtgcag gtccgccacc tggttcatgc ggacttcggc tctaataacg tgctcaccga
     1681 taatgggcgg attactgccg tcatcgactg gtccgaagct atgtttggcg attcgcagta
     1741 cgaagtggcg aacattttct tctggaggcc ttggcttgcc tgcatggaac agcaaacgcg
     1801 ctactttgag cggaggcacc cggagctggc ggggtctccc cgcctcaggg cctatatgtt
     1861 gcggatcggc ctcgaccagc tttaccagtc cctggttgat gggaatttcg acgatgctgc
     1921 ctgggcgcaa ggccgctgtg acgctattgt caggagcggc gcggggaccg tgggccgcac
     1981 tcagatcgcc cggcggtcgg ctgccgtttg gacggatggg tgcgtcgaag tgctcgcgga
     2041 cagcggcaac aggcggcctt ccacccgccc gcgggccaag gagtgaacgc gttcgagtat
     2101 tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt
     2161 ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa
     2221 tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg
     2281 tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc
     2341 aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc
     2401 atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc gggatgcatg
     2461 aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc ggtacccgat
     2521 cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg
     2581 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     2641 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc
     2701 ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga
     2761 cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg
     2821 ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg
     2881 atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc
     2941 tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc
     3001 tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata
     3061 tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg
     3121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3901 gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     4021 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4081 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     4141 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4201 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4261 gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct
     4321 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     4381 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     4441 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     4501 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     4561 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     4621 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     4681 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     4741 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     4801 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     4861 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg
     4921 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     4981 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     5041 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     5101 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     5161 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     5221 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     5281 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg
     5341 gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     5401 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     5461 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     5521 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     5581 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     5641 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     5701 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     5761 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     5821 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     5881 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     5941 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     6001 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     6061 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     6121 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     6181 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     6241 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     6301 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     6361 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     6421 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     6481 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     6541 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     6601 agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     6661 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     6721 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     6781 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     6841 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     6901 acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac
     6961 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     7021 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     7081 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     7141 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     7201 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     7261 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     7321 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     7381 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     7441 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     7501 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     7561 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     7621 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     7681 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     7741 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     7801 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     7861 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     7921 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     7981 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     8041 cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga
     8101 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
     8161 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     8221 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     8281 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     8341 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     8401 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     8461 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     8521 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     8581 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     8641 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     8701 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     8761 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     8821 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     8881 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     8941 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     9001 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     9061 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     9121 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     9181 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     9241 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
     9301 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     9361 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     9421 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     9481 acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac
     9541 tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc
     9601 tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc
     9661 cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg
     9721 cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc
     9781 agcggcggca ggatatattc aattgtaaat ggcttcatgt ccgggaaatc tacatggatc
     9841 agcaatgagt atgatggtca atatggagaa aaagaaagag taattaccaa ttttttttca
     9901 attcaaaaat gtagatgtcc gcagcgttat tataaaatga aagtacattt tgataaaacg
     9961 acaaattacg atccgtcgta tttataggcg aaagcaataa acaaattatt ctaattcgga
    10021 aatctttatt tcga
//
