LOCUS p6ido-Act 10655 bp DNA circular SYN 12-JUN-2013 DEFINITION Ligation of Act-HPT-Xba-Sbf* into pLH9000* ACCESSION p6ido-Act KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10655) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pLH9000* Fragment ends: SbfI and XbaI Fragment size: 7692 Insert molecule: Act-HPT-Xba-Sbf* Fragment ends: XbaI and SbfI Fragment size: 2963 Primer for gene synthesis ActFXba CCCGttctagaCAGGTCGAGGTCAT ActR ccctcgagCTAGCCTAGTACCTACA HPTF ggtactaggctagCTCGAGGGATCCT T35Sbf CTCTAGTGGATCAGCTTGCATGCCT Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC F3F CAGCATTGTTCATCGGTAGT LH1.2 CGAAGGCGGGAAACGACAAT Act1 CACTATCACCGTGAGTTGTC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Border-Left" /SECDrawAs="Region" /SECStyleId=1 /SECName="LB" /SECDescr="Border-Left" misc_feature 313..1710 /gene="pAct" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1741..2310 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2311..2499 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 2500..2955 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2966..3256 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3432..3455 /gene="RB" /product="Border-right" /SECDrawAs="Region" /SECStyleId=1 /SECName="RB" /SECDescr="Border-right" misc_feature 3990..4687 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1 ORI" misc_feature 4724..4983 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5236..8941 /gene="pVS1" /SECDrawAs="Region" /SECStyleId=1 misc_feature 9125..10135 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Sm/Sp" /SECDescr="Strep/Spec resistence" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagggatc ctccacaacc 1741 atgaagaaac cggagctgac cgccacttcc gtcgaaaagt tcctcatcga gaagtttgat 1801 agcgtgtctg accttatgca gttgtcggag ggcgaagagt cccgcgcgtt cagctttgat 1861 gttggcgggc gcgggtacgt cctccgcgtg aactcttgcg ctgacggctt ctataaagat 1921 cggtacgttt acaggcattt cgcctcggcg gccctgccta ttcccgaagt cctcgacatc 1981 ggcgagtttt ccgagagcct tacgtattgt atttcccgcc gggctcaagg ggtgaccctc 2041 caggatctgc cggaaactga gttgcctgcg gttctccagc ccgtcgccga ggctatggac 2101 gccatcgccg cggcggatct ttctcaaacg tcgggcttcg ggccgttcgg ccctcagggg 2161 attggccagt acaccacttg gagggacttt atctgcgcta tcgccgatcc gcacgtgtat 2221 cattggcaaa ccgttatgga cgatacggtc tccgcgagcg tggcccaggc tctggacgaa 2281 ctcatgttgt gggccgagga ttgccccgag gtaagtttct gcttctacct ttgatatata 2341 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa 2401 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat 2461 aacttttcta atatatgacc aaaatttgtt gatgtgcagg tccgccacct ggttcatgcg 2521 gacttcggct ctaataacgt gctcaccgat aatgggcgga ttactgccgt catcgactgg 2581 tccgaagcta tgtttggcga ttcgcagtac gaagtggcga acattttctt ctggaggcct 2641 tggcttgcct gcatggaaca gcaaacgcgc tactttgagc ggaggcaccc ggagctggcg 2701 gggtctcccc gcctcagggc ctatatgttg cggatcggcc tcgaccagct ttaccagtcc 2761 ctggttgatg ggaatttcga cgatgctgcc tgggcgcaag gccgctgtga cgctattgtc 2821 aggagcggcg cggggaccgt gggccgcact cagatcgccc ggcggtcggc tgccgtttgg 2881 acggatgggt gcgtcgaagt gctcgcggac agcggcaaca ggcggccttc cacccgcccg 2941 cgggccaagg agtgaacgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac 3001 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa 3061 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta 3121 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc 3181 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata 3241 tgaggagtaa aacactgaag cctgcaggca tgcaagctga tccactagag gccatggcgg 3301 ccgcactagt ggatcccccg ggatgcatga attcaagctt atcgatgtcg acaggcctta 3361 agggccagat cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac 3421 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga 3481 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat 3541 gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc 3601 aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct 3661 cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc 3721 ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga 3781 tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca 3841 caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact 3901 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt 3961 cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata 4021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 4081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 4261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 4681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga 4801 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 4861 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 4921 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact 4981 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac 5041 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 5101 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggggtac 5161 gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca 5221 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct 5281 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga 5341 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc 5401 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg 5461 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc 5521 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct 5581 ggcccgcagc acccgcgacc tactggacat tgccgagcac atccaggagg ccggcgcggg 5641 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt 5701 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag 5761 cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc 5821 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc 5881 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga 5941 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc 6001 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag 6061 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc 6121 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc 6181 ggtttgtctg atgccaagct cgcggcctgg ccggcgagct tggccgctga agaaaccgag 6241 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct 6301 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg 6361 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg 6421 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc 6481 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga 6541 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc 6601 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg 6661 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc 6721 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag 6781 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg 6841 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc 6901 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta 6961 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct 7021 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga 7081 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa 7141 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca 7201 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag 7261 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag 7321 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg 7381 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat 7441 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg 7501 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat 7561 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa 7621 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag 7681 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga 7741 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat 7801 ggcgagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat 7861 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc 7921 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga 7981 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa 8041 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa 8101 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga 8161 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga 8221 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc 8281 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt 8341 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga 8401 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga 8461 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga 8521 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta 8581 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca 8641 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa 8701 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc 8761 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc 8821 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct 8881 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac 8941 cggtgggtat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga 9001 cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg 9061 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta 9121 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc 9181 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 9241 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 9301 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat 9361 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc 9421 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag 9481 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt 9541 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt 9601 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga 9661 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac 9721 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt 9781 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct 9841 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt 9901 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga 9961 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga 10021 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt 10081 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct 10141 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac 10201 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag 10261 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg 10321 ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg 10381 tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc 10441 gtcggctgat ctcacggatc gactgcttct ctcgcaacgc catccgacgg atgatgttta 10501 aaagtcccat gtggatcact ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac 10561 atggctcagt tctcaatgga aattatctgc ctaaccggct cagttctgcg tagaaaccaa 10621 catgcaagct ccaccgggtg caaagcggca gcggc //