LOCUS       p7i-2x35s-TE9           9557 bp    DNA     circular SYN 12-JUN-2013
DEFINITION  Ligation of 271p6i-2x35s-TE9* into p7iM*
ACCESSION   p7i-2x35s-TE9
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7iM*
               Fragment ends: XhoI and MluI
               Fragment size: 759
            Insert molecule:  271p6i-2x35s-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8798
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    315..1099
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1113..1346
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1347..1535
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1536..1853
                     /gene="bar"
                     /product="basta resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1868..2158
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2334..2357
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2892..3589
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4138..7843
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8027..9037
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      361 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      421 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      481 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      541 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      601 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac
      661 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
      721 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      781 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
      841 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      901 cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg
      961 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     1021 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct
     1081 ctctacaaat ctatctctct cgagtggcca ccatgggccc agaacgacgc ccggccgaca
     1141 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     1201 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     1261 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     1321 gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt ctacctttga tatatatata
     1381 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     1441 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     1501 tttctaatat atgaccaaaa tttgttgatg tgcaggcacg caacgcctac gactggacgg
     1561 ccgagtccac cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct
     1621 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
     1681 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
     1741 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
     1801 acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatc tgagatcacg
     1861 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1921 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1981 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     2041 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     2101 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     2161 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc
     2221 cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag atcttgggcc
     2281 cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga
     2341 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     2401 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     2461 ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga
     2521 aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa
     2581 ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg
     2641 aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc
     2701 ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga
     2761 gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac
     2821 gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga
     2881 tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3661 cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg
     3721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     3781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     3841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     3901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4021 aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc
     4081 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     4141 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     4201 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     4261 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     4321 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     4381 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     4441 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     4501 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     4561 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     4621 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     4681 ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     4741 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     4801 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     4861 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     4921 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     4981 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     5041 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     5101 ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     5161 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     5221 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     5281 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     5341 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     5401 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     5461 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     5521 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     5581 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     5641 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     5701 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     5761 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     5821 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     5881 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     5941 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     6001 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     6061 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     6121 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     6181 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     6241 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     6301 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     6361 tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga
     6421 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     6481 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     6541 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     6601 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     6661 tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta
     6721 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     6781 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     6841 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     6901 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     6961 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     7021 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     7081 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     7141 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     7201 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     7261 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     7321 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     7381 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     7441 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     7501 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     7561 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     7621 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     7681 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     7741 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     7801 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg
     7861 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc
     7921 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     7981 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     8041 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     8101 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     8161 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     8221 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     8281 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     8341 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     8401 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     8461 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     8521 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     8581 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     8641 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     8701 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     8761 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     8821 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     8881 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     8941 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     9001 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     9061 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     9121 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     9181 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg
     9241 aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg
     9301 cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga
     9361 tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca
     9421 ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg
     9481 gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg
     9541 tgcaaagcgg cagcggc
//
