LOCUS       p7i2x-UbiZm            11389 bp    DNA     circular SYN 12-JUN-2013
DEFINITION  Ligation of 594pUbi-BA* into 725p7i-2x35s-TE9*
ACCESSION   p7i2x-UbiZm
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  725p7i-2x35s-TE9*
               Fragment ends: SfiI
               Fragment size: 9493
            Insert molecule:  594pUbi-BA*
               Fragment ends: SfiI
               Fragment size: 1896
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    315..1099
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1113..1346
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1347..1535
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1536..1853
                     /gene="bar"
                     /product="basta resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1868..2158
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2222..3729
                     /gene="'Ubi-int"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3208..3727
                     /gene="int"
                     /product="Intron Ubi"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3790..4043
                     /gene="NOS-T"
                     /product="NOS-T"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4166..4189
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4724..5421
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5970..9675
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9859..10869
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      361 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      421 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      481 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      541 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      601 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac
      661 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
      721 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      781 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
      841 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      901 cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg
      961 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     1021 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct
     1081 ctctacaaat ctatctctct cgagtggcca ccatgggccc agaacgacgc ccggccgaca
     1141 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     1201 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     1261 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     1321 gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt ctacctttga tatatatata
     1381 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     1441 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     1501 tttctaatat atgaccaaaa tttgttgatg tgcaggcacg caacgcctac gactggacgg
     1561 ccgagtccac cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct
     1621 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
     1681 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
     1741 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
     1801 acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatc tgagatcacg
     1861 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1921 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1981 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     2041 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     2101 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     2161 agcctgcagg catgcaagct gatccactag aggccatggg ggccaattta aatggcgtac
     2221 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta
     2281 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta
     2341 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa
     2401 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga
     2461 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt
     2521 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg
     2581 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt
     2641 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata
     2701 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa
     2761 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga
     2821 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
     2881 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
     2941 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
     3001 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
     3061 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     3121 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     3181 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc
     3241 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc
     3301 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg
     3361 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc
     3421 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat
     3481 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     3541 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     3601 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     3661 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     3721 tctgcaggta ctagtggatc ccccgggctg caggaattca agcttacgcg tgtcgactcg
     3781 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     3841 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     3901 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     3961 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4021 gtgtcatcta tgttactaga tcgctcgacg catgctggta cctgacgtct gcggccgcat
     4081 taattaaggc cctaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg
     4141 ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     4201 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     4261 atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat
     4321 aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa
     4381 aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc
     4441 ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct
     4501 gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg
     4561 ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt
     4621 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
     4681 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa
     4741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5521 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5581 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     5641 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5701 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     5761 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5821 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5881 cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc
     5941 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     6001 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     6061 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     6121 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     6181 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     6241 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     6301 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     6361 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     6421 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     6481 gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc
     6541 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     6601 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     6661 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     6721 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     6781 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     6841 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     6901 atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg
     6961 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     7021 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     7081 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     7141 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     7201 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     7261 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     7321 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     7381 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     7441 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     7501 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     7561 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     7621 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     7681 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     7741 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     7801 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     7861 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     7921 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     7981 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     8041 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     8101 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     8161 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg
     8221 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     8281 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     8341 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     8401 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     8461 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     8521 gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     8581 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     8641 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     8701 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     8761 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     8821 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     8881 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     8941 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     9001 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     9061 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     9121 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     9181 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     9241 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     9301 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     9361 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     9421 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     9481 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     9541 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     9601 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     9661 ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
     9721 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
     9781 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     9841 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     9901 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     9961 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
    10021 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
    10081 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
    10141 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
    10201 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
    10261 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
    10321 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
    10381 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
    10441 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
    10501 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
    10561 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
    10621 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
    10681 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
    10741 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
    10801 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
    10861 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
    10921 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
    10981 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
    11041 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc
    11101 gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg
    11161 cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg
    11221 acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg
    11281 gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc
    11341 tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc
//
