LOCUS       p8i2x                  10180 bp    DNA     circular SYN 12-JUN-2013
DEFINITION  Ligation of pmi1-2* into 271p6i-2x35s-TE9*
ACCESSION   p8i2x
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  271p6i-2x35s-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8798
            Insert molecule:  pmi1-2*
               Fragment ends: XhoI and MluI
               Fragment size: 1382
            Sequencing primers	
            35RT	CTTCGCAAGACCCTTCCTCT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    67..851
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             869..1159
                     /gene="ORF-1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQ"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="PMI"
     CDS             1160..1348
                     /gene="int"
                     /product="Intron 'ST-LS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1349..2233
                     /gene="moPMI"
                     /codon_start=2
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2243..2533
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2709..2732
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3267..3964
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4513..8218
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8402..9412
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    9933..9956
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga
       61 tcgggccaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      121 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      181 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
      241 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
      301 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
      361 aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct
      421 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
      481 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
      541 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
      601 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      661 gcatcgtgga aaaagaagac cttccaacca cgtcttcaaa gcaagtggat tgatgtgata
      721 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
      781 tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa
      841 atctatctct ctcgagtcct ccaccaccat gcagaagctc atcaactccg tgcaaaatta
      901 cgcctggggc agcaaaaccg cgctgacgga gttgtatggc atggaaaacc cgtcttcgca
      961 gccaatggct gagctgtgga tgggagccca ccccaagtcc agctctcgcg tgcagaacgc
     1021 ggctggggac attgtttccc tcagggatgt gatcgagtcc gacaagagca cactcctggg
     1081 cgaagccgtc gcgaagcggt tcggcgagtt gccgtttctc ttcaaagttc tgtgtgccgc
     1141 tcaacccctc tccatccagg taagtttctg cttctacctt tgatatatat ataataatta
     1201 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta
     1261 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa
     1321 tatatgacca aaatttgttg atgtgcaggt gcatccaaat aagcacaaca gcgagattgg
     1381 tttcgccaag gaaaatgccg cgggaatccc gatggatgct gcggagcgca actacaagga
     1441 ccccaaccat aaaccagagc tggtctttgc cttgaccccg ttcctcgcta tgaatgcctt
     1501 cagggaattt tctgagatcg tgtcgctgct ccaacccgtt gcgggcgctc acccagccat
     1561 tgcgcatttc ttgcagcagc cggatgccga gcgtctgtcc gaactcttcg cgagcctgct
     1621 caacatgcaa ggggaggaaa agtctcgcgc tctggccatc ttgaagtcgg ccctcgactc
     1681 ccagcagggc gagccgtggc aaacgattag gctcatcagc gagttttacc ccgaagattc
     1741 cggtctgttc agcccactgt tgctcaacgt cgtgaaactg aatccgggcg aggcgatgtt
     1801 tctcttcgct gagaccccac acgcctacct ccagggagtc gcgctggaag ttatggctaa
     1861 ctctgacaat gtgctgcggg ccgggttgac acccaagtat atcgacattc ccgagctggt
     1921 cgcgaacgtg aagttcgagg ccaagcccgc taaccagctc ctcacgcaac cagttaaaca
     1981 gggcgcggaa ctggattttc cgatccccgt ggacgatttc gccttctcgt tgcatgacct
     2041 ctccgataag gagaccacaa tcagccaaca gtctgccgct attctgtttt gcgtcgaggg
     2101 cgacgcgacg ctctggaagg gttcgcagca actccagctg aagccaggag aatccgcctt
     2161 catcgctgcg aatgagagcc cggttaccgt caaagggcac ggtcgcctcg ccagggtcta
     2221 caacaagctg tgacgcgttc gagtattatg gcattgggaa aactgttttt cttgtaccat
     2281 ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg
     2341 gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata
     2401 ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa
     2461 cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga
     2521 ggagtaaaac actgaagcct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     2581 cactagtgga tcccccggga tgcatgaatt caagcttatc gatgtcgaca ggccttaagg
     2641 gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat
     2701 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     2761 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     2821 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac
     2881 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt
     2941 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga
     3001 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct
     3061 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag
     3121 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca
     3181 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc
     3241 cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt tttgataatc
     3301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg cctttgagtg
     4081 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4141 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4201 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     4261 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4321 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4381 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc
     4441 gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     4501 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4561 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     4621 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     4681 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     4741 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     4801 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     4861 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     4921 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     4981 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     5041 gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     5101 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     5161 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     5221 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     5281 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     5341 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     5401 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     5461 ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc
     5521 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     5581 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     5641 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     5701 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     5761 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     5821 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     5881 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     5941 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     6001 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     6061 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     6121 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     6181 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     6241 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     6301 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     6361 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     6421 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     6481 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6541 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     6601 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     6661 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     6721 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg cccagcggca
     6781 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     6841 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     6901 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     6961 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     7021 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     7081 gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     7141 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     7201 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     7261 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     7321 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     7381 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     7441 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     7501 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7561 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     7621 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     7681 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     7741 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     7801 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     7861 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     7921 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     7981 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     8041 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     8101 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     8161 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg
     8221 tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     8281 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     8341 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     8401 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     8461 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     8521 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     8581 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     8641 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     8701 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     8761 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     8821 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     8881 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     8941 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     9001 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     9061 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     9121 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     9181 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     9241 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     9301 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     9361 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     9421 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     9481 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     9541 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     9601 tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg
     9661 gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc
     9721 ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg atgtttaaaa
     9781 gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg
     9841 gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag aaaccaacat
     9901 gcaagctcca ccgggtgcaa agcggcagcg gcggcaggat atattcaatt gtaaatggct
     9961 tcatgtccgg gaaatctaca tggatcagca atgagtatga tggtcaatat ggagaaaaag
    10021 aaagagtaat taccaatttt ttttcaattc aaaaatgtag atgtccgcag cgttattata
    10081 aaatgaaagt acattttgat aaaacgacaa attacgatcc gtcgtattta taggcgaaag
    10141 caataaacaa attattctaa ttcggaaatc tttatttcga
//
