LOCUS       p9i2x                   9805 bp    DNA     circular SYN 05-JUN-2013
DEFINITION  Ligation of NptIIo-Xho-Mlu* into 271p6i-2x35s-TE9*
ACCESSION   p9i2x
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  271p6i-2x35s-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8798
            Insert molecule:  NptIIo-Xho-Mlu*
               Fragment ends: XhoI and MluI
               Fragment size: 1007
            Primer for gene synthesis
            NptXho	CCCGTTctcgagtggccaccatgggcgccTGGATTGAACAAGATGG
            NptMlu	CCCGacgcgtTCAGAAGAACTCGTCAA
            Sequencing primers	
            35RT	CTTCGCAAGACCCTTCCTCT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    315..1099
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1113..1149
                     /gene="NptII"
                     /product="Kanamycin resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin resistance"
     misc_signal     1118
                     /label=mut to C
                     /SECDrawAs="Label"
     misc_signal     1121
                     /label=mut to C
                     /SECDrawAs="Label"
     misc_feature    1150..1338
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1339..2105
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin-Resistenz"
     misc_feature    2116..2406
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2582..2605
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3140..3837
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3874..4133
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4386..8091
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8275..9285
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      361 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      421 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      481 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      541 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      601 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac
      661 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
      721 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      781 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
      841 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      901 cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg
      961 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     1021 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct
     1081 ctctacaaat ctatctctct cgagtggcca ccatgggcgc ctggattgaa caagatggat
     1141 tgcacgcagg taagtttctg cttctacctt tgatatatat ataataatta tcattaatta
     1201 gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt
     1261 gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca
     1321 aaatttgttg atgtgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     1381 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     1441 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     1501 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     1561 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     1621 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     1681 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     1741 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     1801 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     1861 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     1921 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     1981 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2041 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2101 tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt
     2161 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg
     2221 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg
     2281 ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg
     2341 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa
     2401 aacactgaag cctgcaggca tgcaagctga tccactagag gccatggcgg ccgcactagt
     2461 ggatcccccg ggatgcatga attcaagctt atcgatgtcg acaggcctta agggccagat
     2521 cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt
     2581 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     2641 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     2701 gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa
     2761 acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc
     2821 ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg
     2881 cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc
     2941 gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca caggatgatc
     3001 tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat
     3061 atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac
     3121 gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3901 ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga gtgagctgat
     3961 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4021 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     4081 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     4141 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     4201 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4261 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggggtac gtcgaggtcg
     4321 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     4381 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     4441 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     4501 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     4561 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     4621 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     4681 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     4741 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     4801 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     4861 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     4921 gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     4981 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     5041 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     5101 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     5161 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     5221 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     5281 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     5341 atgccaagct cgcggcctgg ccggcgagct tggccgctga agaaaccgag cgccgccgtc
     5401 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     5461 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     5521 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     5581 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     5641 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     5701 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     5761 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     5821 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     5881 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     5941 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     6001 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     6061 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     6121 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     6181 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     6241 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     6301 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     6361 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     6421 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     6481 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     6541 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     6601 ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc
     6661 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     6721 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     6781 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     6841 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     6901 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggcgagtgtg
     6961 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     7021 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     7081 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     7141 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     7201 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     7261 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     7321 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     7381 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     7441 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     7501 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     7561 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     7621 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     7681 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     7741 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     7801 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     7861 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     7921 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     7981 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     8041 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cggtgggtat
     8101 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     8161 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     8221 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     8281 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     8341 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     8401 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     8461 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     8521 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     8581 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     8641 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     8701 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     8761 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     8821 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     8881 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     8941 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     9001 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     9061 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     9121 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     9181 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     9241 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt
     9301 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     9361 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     9421 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat
     9481 agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat
     9541 tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat
     9601 ctcacggatc gactgcttct ctcgcaacgc catccgacgg atgatgttta aaagtcccat
     9661 gtggatcact ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt
     9721 tctcaatgga aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct
     9781 ccaccgggtg caaagcggca gcggc
//
