LOCUS       p75N                    8783 bp    DNA     circular SYN 02-JUN-2006
DEFINITION  Ligation of p95P-Nos* into pLH7500*
ACCESSION   p75N
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pLH7500*
               Fragment ends: XhoI and XbaI
               Fragment size: 8479
            Insert molecule:  p95P-Nos*
               Fragment ends: XbaI and XhoI
               Fragment size: 304
FEATURES             Location/Qualifiers
     misc_feature    7..304
                     /gene="P-NOS"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    319..870
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    893..1093
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    1311
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Marker"
     misc_feature    1816..2513
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3062..6767
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     misc_feature    6951..7961
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Region"
     misc_feature    8495
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Marker"
ORIGIN      
        1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
       61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      301 gcacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc
      361 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
      421 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
      481 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
      541 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc
      601 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg
      661 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg
      721 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag
      781 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc
      841 cgtccggtcc tgcccgtcac cgagatctga gatcacgcgt tctagtccgc aaaaatcacc
      901 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
      961 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     1021 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     1081 ccaaaatcca gtgacctgca ggcatgcaag ctagaggccc ttaaggcctt actagtggat
     1141 cccccgggat gcatgaattc aagcttatcg atgtcgacgc ggccgccatg gccagatctg
     1201 ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac
     1261 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     1321 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     1381 tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt
     1441 cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct
     1501 agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc
     1561 gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc
     1621 tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc
     1681 gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc
     1741 gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg
     1801 ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2041 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2581 cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga gctgataccg
     2641 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2701 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2761 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2821 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2881 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2941 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc
     3001 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     3061 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     3121 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     3181 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     3241 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     3301 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     3361 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     3421 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     3481 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     3541 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     3601 ccaaggcgcg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     3661 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     3721 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     3781 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     3841 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     3901 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     3961 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     4021 caagctcgcg gcctggccgg cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     4081 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     4141 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     4201 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     4261 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     4321 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     4381 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     4441 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     4501 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     4561 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     4621 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     4681 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     4741 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     4801 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     4861 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     4921 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     4981 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     5041 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     5101 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     5161 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     5221 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5281 gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg
     5341 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     5401 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     5461 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     5521 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     5581 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcg agtgtgtggg
     5641 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     5701 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     5761 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     5821 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     5881 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     5941 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6001 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6061 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6121 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6181 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6241 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6301 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     6361 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     6421 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     6481 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     6541 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     6601 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     6661 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     6721 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccggt gggtatgcct
     6781 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga
     6841 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     6901 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6961 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     7021 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     7081 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     7141 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     7201 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     7261 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     7321 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     7381 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     7441 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     7501 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     7561 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     7621 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7681 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7741 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7801 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7861 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7921 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa
     7981 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     8041 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     8101 cctacacaaa ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt
     8161 ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta
     8221 cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca
     8281 cggatcgact gcttctctcg caacgccatc cgacggatga tgtttaaaag tcccatgtgg
     8341 atcactccgt tgccccgtcg ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc
     8401 aatggaaatt atctgcctaa ccggctcagt tctgcgtaga aaccaacatg caagctccac
     8461 cgggtgcaaa gcggcagcgg cggcaggata tattcaattg taaatggctt catgtccggg
     8521 aaatctacat ggatcagcaa tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt
     8581 accaattttt tttcaattca aaaatgtaga tgtccgcagc gttattataa aatgaaagta
     8641 cattttgata aaacgacaaa ttacgatccg tcgtatttat aggcgaaagc aataaacaaa
     8701 ttattctaat tcggaaatct ttatttcgac gtgtctacat tcacgtccaa atgggggctt
     8761 agatgagaaa cttcacgatc ggc
//
