LOCUS       p7L                     8981 bp    DNA     circular SYN 28-SEP-2006
DEFINITION  Ligation of pLH7000* into p7L-int*
ACCESSION   p7L
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7L-int*
               Fragment ends: MluI and XhoI
               Fragment size: 8411
            Insert molecule:  pLH7000*
               Fragment ends: XhoI and MluI
               Fragment size: 570
FEATURES             Location/Qualifiers
     CDS             7..40
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Gene"
     misc_feature    41..456
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     misc_feature    473..1024
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    1046..1247
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1251..1284
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Region"
     misc_feature    1456..1479
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    2014..2711
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3260..6965
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             7149..8159
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    8680..8703
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaataa cttcgtataa tgtatactat acgaagttat acatggtgga gcacgacact
       61 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact
      121 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      181 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa
      241 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc
      301 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      361 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      421 tcctctatat aaggaagttc atttcatttg gagaggacct cgagtggcca ccatgggccc
      481 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac
      541 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc
      601 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga
      661 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta
      721 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg
      781 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt
      841 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc
      901 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt
      961 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat
     1021 ctgagatcac gcgttctagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc
     1081 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     1141 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     1201 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc ataacttcgt
     1261 ataatgtata ctatacgaag ttatcctgca ggcatgcaag ctgatccact agaggccatg
     1321 gcggccgcac tagtggatcc cccgggatgc atgaattcaa gcttatcgat gtcgacaggc
     1381 cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt
     1441 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     1501 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     1561 gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct
     1621 gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt
     1681 ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg
     1741 tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc
     1801 gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc
     1861 gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg
     1921 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg
     1981 cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt
     2041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct
     2821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2881 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2941 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3001 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3061 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3121 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     3181 gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     3241 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     3301 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     3361 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     3421 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     3481 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     3541 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     3601 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     3661 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     3721 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     3781 ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca
     3841 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     3901 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     3961 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     4021 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     4081 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     4141 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     4201 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac
     4261 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     4321 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     4381 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     4441 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     4501 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     4561 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     4621 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     4681 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     4741 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     4801 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     4861 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     4921 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     4981 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     5041 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     5101 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     5161 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     5221 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     5281 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     5341 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     5401 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     5461 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc
     5521 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5581 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5641 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5701 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5761 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5821 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5881 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5941 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     6001 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     6061 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     6121 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     6181 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     6241 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     6301 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6361 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6421 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6481 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6541 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6601 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6661 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6721 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6781 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6841 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6901 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6961 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
     7021 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     7081 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     7141 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     7201 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     7261 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     7321 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     7381 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     7441 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     7501 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     7561 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     7621 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     7681 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     7741 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     7801 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     7861 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     7921 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     7981 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     8041 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     8101 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     8161 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     8221 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     8281 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt
     8341 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg
     8401 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg
     8461 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg
     8521 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt
     8581 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa
     8641 ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta
     8701 aatggcttca tgtccgggaa atctacatgg atcagcaatg agtatgatgg tcaatatgga
     8761 gaaaaagaaa gagtaattac caattttttt tcaattcaaa aatgtagatg tccgcagcgt
     8821 tattataaaa tgaaagtaca ttttgataaa acgacaaatt acgatccgtc gtatttatag
     8881 gcgaaagcaa taaacaaatt attctaattc ggaaatcttt atttcgacgt gtctacattc
     8941 acgtccaaat gggggcttag atgagaaact tcacgatcgg c
//
