LOCUS       p7N-35S                 9419 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  Ligation of p35OCS-BM* into p7N*
ACCESSION   p7N-35S
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7N*
               Fragment ends: SfiI
               Fragment size: 8727
            Insert molecule:  p35OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
            XbaI is normally methylated
FEATURES             Location/Qualifiers
     misc_feature    7..304
                     /gene="P-NOS"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    319..870
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    893..1093
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    1145..1562
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     CDS             1533..1539
                     /gene="(null)"
                     /product="TATA-box"
                     /SECDrawAs="Gene"
     misc_feature    1614..1809
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    1894..1917
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    2452..3149
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3698..7403
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             7587..8597
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9118..9141
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
       61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      301 gcacctcgag tggccaccat gggcccagaa cgacgcccgg ccgacatccg ccgtgccacc
      361 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
      421 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
      481 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
      541 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc
      601 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg
      661 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg
      721 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag
      781 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc
      841 cgtccggtcc tgcccgtcac cgagatctga gatcacgcgt tctagtccgc aaaaatcacc
      901 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
      961 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     1021 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     1081 ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac
     1141 tagatcaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     1201 tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     1261 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc
     1321 ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctaccgac
     1381 agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca
     1441 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     1501 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     1561 aggacactag ttggatcccc cgggctgcag gaattcaagc ttacgcgtgt cgaccctgct
     1621 ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca
     1681 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct
     1741 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac
     1801 tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccga tcagattgtc
     1861 gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     1921 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     1981 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc
     2041 cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc
     2101 attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg
     2161 cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat
     2221 ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc
     2281 atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca
     2341 atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc
     2401 atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc
     2461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2701 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3241 gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3301 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3361 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3421 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     3481 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3541 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3601 gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag
     3661 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     3721 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     3781 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     3841 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     3901 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     3961 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     4021 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     4081 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     4141 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     4201 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg
     4261 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     4321 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     4381 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     4441 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     4501 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     4561 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     4621 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag
     4681 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     4741 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     4801 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     4861 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     4921 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     4981 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     5041 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     5101 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     5161 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     5221 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     5281 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     5341 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     5401 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     5461 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     5521 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     5581 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     5641 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     5701 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     5761 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     5821 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     5881 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     5941 aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     6001 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     6061 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     6121 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     6181 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     6241 tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg
     6301 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     6361 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     6421 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     6481 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     6541 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     6601 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     6661 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     6721 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     6781 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     6841 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     6901 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     6961 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     7021 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     7081 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     7141 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     7201 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     7261 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     7321 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     7381 gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct
     7441 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
     7501 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     7561 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     7621 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     7681 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     7741 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     7801 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7861 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7921 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7981 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     8041 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     8101 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     8161 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     8221 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     8281 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     8341 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     8401 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     8461 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     8521 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     8581 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
     8641 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     8701 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     8761 atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt
     8821 gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc
     8881 gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac
     8941 gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca
     9001 ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg
     9061 ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc
     9121 aggatatatt caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag
     9181 tatgatggtc aatatggaga aaaagaaaga gtaattacca attttttttc aattcaaaaa
     9241 tgtagatgtc cgcagcgtta ttataaaatg aaagtacatt ttgataaaac gacaaattac
     9301 gatccgtcgt atttataggc gaaagcaata aacaaattat tctaattcgg aaatctttat
     9361 ttcgacgtgt ctacattcac gtccaaatgg gggcttagat gagaaacttc acgatcggc
//
