LOCUS       p7U                    10003 bp    DNA     circular SYN 31-MAY-2002
DEFINITION  Ligation of pUbi-ABM into pLH7000*
ACCESSION   p7U
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             3..26
                     /gene="'UF-Spe"
                     /product="Primer UF-Spe"
                     /SECDrawAs="Gene"
     CDS             10..1517
                     /gene="Ubi-int'"
                     /product="Promotor"
                     /SECDrawAs="Gene"
     CDS             575..594
                     /gene="UF1"
                     /product="Primer UF1seq 584"
                     /SECDrawAs="Gene"
     misc_feature    997..1516
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
     CDS             1468..1486
                     /gene="(null)"
                     /product="Primer Ubi1"
                     /SECDrawAs="Gene"
     CDS             complement (1504..1515)
                     /gene="'UR-Sal"
                     /product="Primer-UR-Sal"
                     /SECDrawAs="Gene"
     CDS             1531..2082
                     /gene="pat"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
     misc_feature    2105..2305
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2478..2501
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    3036..3733
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4282..7987
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8171..9181
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9702..9725
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtagtc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc
      661 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
      721 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
      781 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga
      841 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc
      901 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc
      961 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc
     1021 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta
     1081 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt
     1141 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt
     1201 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag gataggtata
     1261 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata
     1321 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga
     1381 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc
     1441 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt
     1501 ggtgttactt ctgcaggtcg agtggccacc atgggcccag aacgacgccc ggccgacatc
     1561 cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag
     1621 acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc
     1681 gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc
     1741 atcgcctacg cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc
     1801 gtgtacgtct ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg
     1861 ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac
     1921 gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg
     1981 gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg
     2041 ccggtaccgc cccgtccggt cctgcccgtc accgagatct gagatcacgc gttctagtcc
     2101 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     2161 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     2221 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2281 taattcctaa aaccaaaatc cagtgacctg caggcatgca agctgatcca ctagaggcca
     2341 tggcggccgc actagtggat cccccgggat gcatgaattc aagcttatcg atgtcgacag
     2401 gccttaaggg ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt
     2461 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2521 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     2581 gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt
     2641 ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc
     2701 ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc
     2761 ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa
     2821 gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt
     2881 ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg
     2941 ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg
     3001 ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg aagatccttt
     3061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgattaccgc
     3841 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3901 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3961 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     4021 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     4081 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4141 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     4201 gggtacgtcg aggtcgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     4261 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     4321 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     4381 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     4441 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     4501 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     4561 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     4621 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     4681 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     4741 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     4801 ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc gccctaccct
     4861 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     4921 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     4981 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     5041 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     5101 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     5161 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     5221 ctggccggtt tgtctgatgc caagctcgcg gcctggccgg cgagcttggc cgctgaagaa
     5281 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     5341 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     5401 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     5461 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     5521 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     5581 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     5641 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     5701 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     5761 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     5821 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     5881 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     5941 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     6001 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     6061 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     6121 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     6181 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     6241 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     6301 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     6361 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     6421 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     6481 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc
     6541 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     6601 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     6661 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     6721 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     6781 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     6841 cggcatggcg agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     6901 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     6961 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     7021 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     7081 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     7141 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     7201 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     7261 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     7321 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     7381 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     7441 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     7501 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     7561 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     7621 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     7681 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     7741 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     7801 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     7861 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     7921 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     7981 aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag
     8041 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     8101 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     8161 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     8221 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     8281 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     8341 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     8401 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     8461 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     8521 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     8581 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     8641 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     8701 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     8761 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     8821 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     8881 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     8941 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     9001 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     9061 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     9121 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     9181 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     9241 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     9301 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     9361 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg
     9421 tgaacgtcgg ctcgattgta cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc
     9481 cggtgcgtcg gctgatctca cggatcgact gcttctctcg caacgccatc cgacggatga
     9541 tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt tggggggaag
     9601 gtgcacatgg ctcagttctc aatggaaatt atctgcctaa ccggctcagt tctgcgtaga
     9661 aaccaacatg caagctccac cgggtgcaaa gcggcagcgg cggcaggata tattcaattg
     9721 taaatggctt catgtccggg aaatctacat ggatcagcaa tgagtatgat ggtcaatatg
     9781 gagaaaaaga aagagtaatt accaattttt tttcaattca aaaatgtaga tgtccgcagc
     9841 gttattataa aatgaaagta cattttgata aaacgacaaa ttacgatccg tcgtatttat
     9901 aggcgaaagc aataaacaaa ttattctaat tcggaaatct ttatttcgac gtgtctacat
     9961 tcacgtccaa atgggggctt agatgagaaa cttcacgatc ggc
//
