LOCUS       p7Lox-int               9170 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  Ligation of bar-int-Lox-Xba-Sbf* into p7int*
ACCESSION   p7Lox-int
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7int*
               Fragment ends: SbfI and XbaI
               Fragment size: 7692
            Insert molecule:  bar-int-Lox-Xba-Sbf*
               Fragment ends: XbaI and SbfI
               Fragment size: 1478
FEATURES             Location/Qualifiers
     CDS             7..40
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Gene"
     misc_feature    41..456
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     misc_feature    473..706
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    707..895
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Region"
     misc_feature    896..1213
                     /gene="bar"
                     /product="basta resistance"
                     /SECDrawAs="Region"
     misc_feature    1235..1436
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     CDS             1440..1473
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Gene"
     misc_feature    1645..1668
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    2203..2900
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3449..7154
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             7338..8348
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    8869..8892
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaataa cttcgtataa tgtatactat acgaagttat acatggtgga gcacgacact
       61 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact
      121 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      181 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa
      241 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc
      301 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      361 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      421 tcctctatat aaggaagttc atttcatttg gagaggacct cgagtggcca ccatgggccc
      481 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac
      541 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc
      601 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga
      661 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt
      721 ctacctttga tatatatata ataattatca ttaattagta gtaatataat atttcaaata
      781 tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt
      841 gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggcacg
      901 caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac
      961 gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa
     1021 gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct
     1081 cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga
     1141 cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg tcctgcccgt
     1201 caccgagatc tgagatcacg cgttctagtc cgcaaaaatc accagtctct ctctacaaat
     1261 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
     1321 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     1381 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacca
     1441 taacttcgta taatgtatac tatacgaagt tatcctgcag gcatgcaagc tgatccacta
     1501 gaggccatgg cggccgcact agtggatccc ccgggatgca tgaattcaag cttatcgatg
     1561 tcgacaggcc ttaagggcca gatcttgggc ccggtacccg atcagattgt cgtttcccgc
     1621 cttcggttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     1681 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     1741 tttgtatgtg catgccaacc acagggttcc cctcgggagt gcttggcatt ccgtgcgata
     1801 atgacttctg ttcaaccacc caaacgtcgg aaagcctgac gacggagcag cattccaaaa
     1861 agatcccttg gctcgtctgg gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc
     1921 tcaacgcggt cgcggacgta gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg
     1981 tcgaaaagcg tgatctgctt gtcgctcttt cggccgacgt cctggccagt catcacgcgc
     2041 caaagttccg tcacaggatg atctggcgcg agttgctgga tctcgccttc aatccgggtc
     2101 tgtggcggga actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc
     2161 ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc cgaaaaggat ctaggtgaag
     2221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccga
     3001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3061 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     3121 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3181 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     3241 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3301 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3361 gaggcagggg tacgtcgagg tcgatccaac ccctccgctg ctatagtgca gtcggcttct
     3421 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     3481 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     3541 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     3601 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     3661 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     3721 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     3781 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     3841 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     3901 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     3961 accgcacccg gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc
     4021 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     4081 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     4141 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     4201 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     4261 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     4321 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     4381 tgaaatcctg gccggtttgt ctgatgccaa gctcgcggcc tggccggcga gcttggccgc
     4441 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     4501 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     4561 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     4621 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     4681 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     4741 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     4801 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4861 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4921 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4981 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     5041 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     5101 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     5161 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     5221 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     5281 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     5341 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     5401 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     5461 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     5521 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     5581 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     5641 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggcggcgc
     5701 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5761 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5821 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5881 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5941 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     6001 ggccggccgg catggcgagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     6061 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     6121 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     6181 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     6241 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     6301 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     6361 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6421 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6481 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6541 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6601 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6661 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6721 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6781 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6841 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     6901 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     6961 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     7021 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     7081 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     7141 cacatcaagg caccggtggg tatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt
     7201 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
     7261 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     7321 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     7381 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     7441 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     7501 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     7561 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     7621 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     7681 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     7741 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     7801 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     7861 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     7921 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     7981 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     8041 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     8101 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     8161 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     8221 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     8281 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     8341 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     8401 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     8461 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc
     8521 tggctttttc ttgttatcgc aatagttggc gaagtaatcg caacatagct tgcttggtcg
     8581 ttccgcgtga acgtcggctc gattgtacct gcgttcaaat actttgcgat cgtgttgcgc
     8641 gcctgcccgg tgcgtcggct gatctcacgg atcgactgct tctctcgcaa cgccatccga
     8701 cggatgatgt ttaaaagtcc catgtggatc actccgttgc cccgtcgctc accgtgttgg
     8761 ggggaaggtg cacatggctc agttctcaat ggaaattatc tgcctaaccg gctcagttct
     8821 gcgtagaaac caacatgcaa gctccaccgg gtgcaaagcg gcagcggcgg caggatatat
     8881 tcaattgtaa atggcttcat gtccgggaaa tctacatgga tcagcaatga gtatgatggt
     8941 caatatggag aaaaagaaag agtaattacc aatttttttt caattcaaaa atgtagatgt
     9001 ccgcagcgtt attataaaat gaaagtacat tttgataaaa cgacaaatta cgatccgtcg
     9061 tatttatagg cgaaagcaat aaacaaatta ttctaattcg gaaatcttta tttcgacgtg
     9121 tctacattca cgtccaaatg ggggcttaga tgagaaactt cacgatcggc
//
