LOCUS       p7int-Ubi              10192 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  Ligation of Ubi into p7int*
ACCESSION   p7int-Ubi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Restriction of pUbi-ABM with Spe-Sal and cloning of the Ubi-promotor into the Xba-Xho sites of p7int. Exchange of promotor.
FEATURES             Location/Qualifiers
     misc_feature    10..1517
                     /gene="Ubi-int'"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    997..1516
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
     misc_feature    1531..1764
                     /gene="pat"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    1765..1953
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Region"
     misc_feature    1954..2271
                     /gene="pat"
                     /product="basta resistance"
                     /SECDrawAs="Region"
     misc_feature    2293..2494
                     /gene="T35S"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2667..2690
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    3225..3922
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4471..8176
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8360..9370
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9891..9914
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtagtc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc
      661 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
      721 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
      781 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga
      841 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc
      901 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc
      961 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc
     1021 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta
     1081 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt
     1141 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt
     1201 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag gataggtata
     1261 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata
     1321 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga
     1381 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc
     1441 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt
     1501 ggtgttactt ctgcaggtcg agtggccacc atgggcccag aacgacgccc ggccgacatc
     1561 cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag
     1621 acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc
     1681 gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc
     1741 atcgcctacg cgggcccctg gaaggtaagt ttctgcttct acctttgata tatatataat
     1801 aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg
     1861 tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt
     1921 tctaatatat gaccaaaatt tgttgatgtg caggcacgca acgcctacga ctggacggcc
     1981 gagtcgaccg tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac
     2041 acccacctgc tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg
     2101 ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg
     2161 ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac
     2221 ttcagcctgc cggtaccgcc ccgtccggtc ctgcccgtca ccgagatctg agatcacgcg
     2281 ttctagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     2341 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     2401 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     2461 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcaa gctgatccac
     2521 tagaggccat ggcggccgca ctagtggatc ccccgggatg catgaattca agcttatcga
     2581 tgtcgacagg ccttaagggc cagatcttgg gcccggtacc cgatcagatt gtcgtttccc
     2641 gccttcggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     2701 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     2761 catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgcttggca ttccgtgcga
     2821 taatgacttc tgttcaacca cccaaacgtc ggaaagcctg acgacggagc agcattccaa
     2881 aaagatccct tggctcgtct gggtcggcta gaaggtcgag tgggctgctg tggcttgatc
     2941 cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca aatccgacgc
     3001 tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca gtcatcacgc
     3061 gccaaagttc cgtcacagga tgatctggcg cgagttgctg gatctcgcct tcaatccggg
     3121 tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg
     3181 tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgaaaagg atctaggtga
     3241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3481 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3721 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3781 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3901 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4021 gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4081 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4141 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4201 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     4261 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4321 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4381 gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc tgctatagtg cagtcggctt
     4441 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     4501 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     4561 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     4621 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     4681 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     4741 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     4801 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     4861 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     4921 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     4981 cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga ggcgtgaagt ttggcccccg
     5041 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     5101 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     5161 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     5221 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     5281 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     5341 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     5401 catgaaatcc tggccggttt gtctgatgcc aagctcgcgg cctggccggc gagcttggcc
     5461 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     5521 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     5581 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     5641 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     5701 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     5761 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     5821 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     5881 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     5941 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     6001 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     6061 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     6121 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     6181 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     6241 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     6301 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     6361 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     6421 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     6481 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     6541 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     6601 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     6661 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggcggc
     6721 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     6781 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     6841 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     6901 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     6961 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     7021 agggccggcc ggcatggcga gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     7081 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     7141 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     7201 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     7261 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     7321 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     7381 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     7441 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     7501 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     7561 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     7621 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     7681 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     7741 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg
     7801 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
     7861 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
     7921 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
     7981 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
     8041 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
     8101 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
     8161 cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg gagaccgaaa ccttgcgctc
     8221 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     8281 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     8341 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     8401 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     8461 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     8521 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     8581 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     8641 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     8701 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     8761 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     8821 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     8881 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     8941 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     9001 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     9061 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     9121 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     9181 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     9241 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     9301 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     9361 cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     9421 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     9481 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgaaag
     9541 gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatag cttgcttggt
     9601 cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg atcgtgttgc
     9661 gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc aacgccatcc
     9721 gacggatgat gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc tcaccgtgtt
     9781 ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac cggctcagtt
     9841 ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc ggcaggatat
     9901 attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat gagtatgatg
     9961 gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa aaatgtagat
    10021 gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat tacgatccgt
    10081 cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt tatttcgacg
    10141 tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg gc
//
