LOCUS       p6-35S-OCS             10029 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  Ligation of 35S-OCS-BM into pLH6000*
ACCESSION   p6-35S-OCS
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|GenBank            10029 bp    DNA     circular SYN 19-MAR-
            2003
            ACCESSION   p6-35S-OCS
            KEYWORDS:   . 
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     CDS             452..1477
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
     misc_feature    1502..1703
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1755..2172
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     CDS             2143..2149
                     /gene="(null)"
                     /product="TATA-box"
                     /SECDrawAs="Gene"
     misc_feature    2224..2419
                     /gene="TOCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2504..2527
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
     misc_feature    3062..3759
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4308..8013
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8197..9207
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9728..9751
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag ggccccgggg ggcaataaga tatgaaaaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      901 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      961 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1021 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1081 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1141 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1201 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1261 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1321 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1381 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1441 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cggagtccgc
     1501 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     1561 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     1621 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     1681 attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccatg
     1741 gcggccgcac tagatcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     1801 agatacagtc tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg
     1861 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     1921 ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     1981 ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga
     2041 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     2101 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2161 tcatttggag aggacactag ttggatcccc cgggctgcag gaattcaagc ttacgcgtgt
     2221 cgaccctgct ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt
     2281 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
     2341 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
     2401 ttcaatttac tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccga
     2461 tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg
     2521 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     2581 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg
     2641 cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg
     2701 acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg
     2761 gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc
     2821 gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc
     2881 ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat
     2941 ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat
     3001 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc
     3061 gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3721 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3781 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3841 tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3961 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     4021 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     4081 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4141 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4201 cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc
     4261 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     4321 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     4381 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     4441 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     4501 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     4561 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     4621 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     4681 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     4741 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     4801 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc
     4861 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     4921 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     4981 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     5041 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     5101 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     5161 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     5221 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct
     5281 ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     5341 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     5401 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     5461 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     5521 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     5581 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     5641 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     5701 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     5761 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     5821 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     5881 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     5941 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     6001 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     6061 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     6121 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     6181 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     6241 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     6301 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     6361 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     6421 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     6481 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     6541 gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     6601 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     6661 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     6721 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     6781 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     6841 cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta
     6901 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     6961 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     7021 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     7081 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     7141 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     7201 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     7261 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     7321 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     7381 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     7441 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     7501 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     7561 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     7621 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     7681 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     7741 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     7801 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     7861 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     7921 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     7981 ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag
     8041 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
     8101 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     8161 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     8221 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     8281 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     8341 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     8401 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     8461 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     8521 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     8581 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     8641 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     8701 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     8761 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     8821 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     8881 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     8941 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     9001 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     9061 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     9121 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     9181 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     9241 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     9301 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     9361 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc
     9421 aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata
     9481 ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt
     9541 ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc
     9601 ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct
     9661 gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg
     9721 cagcggcggc aggatatatt caattgtaaa tggcttcatg tccgggaaat ctacatggat
     9781 cagcaatgag tatgatggtc aatatggaga aaaagaaaga gtaattacca attttttttc
     9841 aattcaaaaa tgtagatgtc cgcagcgtta ttataaaatg aaagtacatt ttgataaaac
     9901 gacaaattac gatccgtcgt atttataggc gaaagcaata aacaaattat tctaattcgg
     9961 aaatctttat ttcgacgtgt ctacattcac gtccaaatgg gggcttagat gagaaacttc
    10021 acgatcggc
//
