LOCUS       p6N-35S                 9909 bp    DNA     circular SYN 02-NOV-2007
DEFINITION  Ligation of p35OCS-BM* into p6N*
ACCESSION   p6N-35S
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p6N*
               Fragment ends: SfiI
               Fragment size: 9217
            Insert molecule:  p35OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
FEATURES             Location/Qualifiers
     misc_feature    7..304
                     /gene="P-NOS"
                     /product="Promotor"
                     /SECDrawAs="Region"
     CDS             332..1357
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
     misc_feature    1382..1583
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1635..2052
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     CDS             2023..2029
                     /gene="(null)"
                     /product="TATA-box"
                     /SECDrawAs="Gene"
     misc_feature    2104..2299
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2384..2407
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
     misc_feature    2942..3639
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4188..7893
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8077..9087
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9608..9631
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
       61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      301 gcacctcgag ggccccgggg ggcaataaga tatgaaaaag cctgaactca ccgcgacgtc
      361 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      421 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      481 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      541 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      601 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      661 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      721 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      781 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      841 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
      901 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
      961 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1021 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1081 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1141 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1201 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1261 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1321 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cggagtccgc
     1381 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     1441 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     1501 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     1561 attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctgatccact agaggccatg
     1621 gcggccgcac tagatcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     1681 agatacagtc tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg
     1741 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     1801 ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     1861 ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga
     1921 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     1981 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2041 tcatttggag aggacactag ttggatcccc cgggctgcag gaattcaagc ttacgcgtgt
     2101 cgaccctgct ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt
     2161 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
     2221 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
     2281 ttcaatttac tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccga
     2341 tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg
     2401 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     2461 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg
     2521 cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg
     2581 acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg
     2641 gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc
     2701 gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc
     2761 ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat
     2821 ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat
     2881 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc
     2941 gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3721 tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3841 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     3901 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     3961 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4021 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4081 cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc
     4141 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     4201 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     4261 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     4321 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     4381 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     4441 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     4501 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     4561 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     4621 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     4681 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc
     4741 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     4801 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     4861 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     4921 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     4981 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     5041 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     5101 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct
     5161 ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     5221 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     5281 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     5341 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     5401 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     5461 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     5521 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     5581 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     5641 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     5701 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     5761 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     5821 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     5881 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     5941 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     6001 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     6061 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     6121 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     6181 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     6241 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     6301 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     6361 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     6421 gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     6481 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     6541 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     6601 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     6661 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     6721 cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta
     6781 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     6841 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     6901 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     6961 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     7021 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     7081 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     7141 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     7201 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     7261 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     7321 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     7381 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     7441 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     7501 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     7561 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     7621 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     7681 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     7741 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     7801 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     7861 ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag
     7921 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
     7981 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     8041 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     8101 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     8161 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     8221 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     8281 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     8341 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     8401 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     8461 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     8521 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     8581 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     8641 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     8701 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     8761 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     8821 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     8881 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     8941 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     9001 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     9061 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     9121 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     9181 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     9241 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc
     9301 aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata
     9361 ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt
     9421 ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc
     9481 ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct
     9541 gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg
     9601 cagcggcggc aggatatatt caattgtaaa tggcttcatg tccgggaaat ctacatggat
     9661 cagcaatgag tatgatggtc aatatggaga aaaagaaaga gtaattacca attttttttc
     9721 aattcaaaaa tgtagatgtc cgcagcgtta ttataaaatg aaagtacatt ttgataaaac
     9781 gacaaattac gatccgtcgt atttataggc gaaagcaata aacaaattat tctaattcgg
     9841 aaatctttat ttcgacgtgt ctacattcac gtccaaatgg gggcttagat gagaaacttc
     9901 acgatcggc
//
