LOCUS       p6U                    10493 bp    DNA     circular SYN 22-JUN-2007
DEFINITION  Ligation of pUbi-ABM* into pLH6000*
ACCESSION   p6U
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             3..26
                     /gene="'UF-Spe"
                     /product="Primer UF-Spe"
                     /SECDrawAs="Gene"
     CDS             10..1517
                     /gene="Ubi-int'"
                     /product="Promotor"
                     /SECDrawAs="Gene"
     CDS             575..594
                     /gene="UF1"
                     /product="Primer UF1seq 584"
                     /SECDrawAs="Gene"
     misc_feature    997..1516
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
     CDS             1468..1486
                     /gene="(null)"
                     /product="Primer Ubi1"
                     /SECDrawAs="Gene"
     CDS             complement (1504..1515)
                     /gene="'UR-Sal"
                     /product="Primer-UR-Sal"
                     /SECDrawAs="Gene"
     CDS             1544..2569
                     /gene="hpt"
                     /product="Hygromycin"
                     /SECDrawAs="Gene"
     misc_feature    2594..2795
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    2968..2991
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
     misc_feature    3526..4223
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4772..8477
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8661..9671
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    10192..10215
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtagtc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc
      661 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
      721 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
      781 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga
      841 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc
      901 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc
      961 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc
     1021 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta
     1081 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt
     1141 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt
     1201 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag gataggtata
     1261 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata
     1321 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga
     1381 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc
     1441 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt
     1501 ggtgttactt ctgcaggtcg agggccccgg ggggcaataa gatatgaaaa agcctgaact
     1561 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     1621 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     1681 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     1741 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     1801 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     1861 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     1921 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     1981 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     2041 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     2101 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     2161 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     2221 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     2281 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     2341 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     2401 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     2461 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     2521 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg
     2581 accggagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc
     2641 cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc
     2701 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     2761 ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgca agctgatcca
     2821 ctagaggcca tggcggccgc actagtggat cccccgggat gcatgaattc aagcttatcg
     2881 atgtcgacag gccttaaggg ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc
     2941 cgccttcggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     3001 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
     3061 ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg
     3121 ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca
     3181 aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat
     3241 ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg
     3301 ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg
     3361 cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg
     3421 gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg
     3481 gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg
     3541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3781 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4321 cgattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4381 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     4441 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4501 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4561 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4621 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4681 cgcgaggcag gggtacgtcg aggtcgatcc aacccctccg ctgctatagt gcagtcggct
     4741 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
     4801 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
     4861 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
     4921 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
     4981 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
     5041 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
     5101 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
     5161 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
     5221 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
     5281 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc
     5341 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     5401 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     5461 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     5521 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     5581 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     5641 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     5701 gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg gcctggccgg cgagcttggc
     5761 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     5821 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     5881 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     5941 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     6001 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     6061 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     6121 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     6181 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     6241 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     6301 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     6361 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     6421 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     6481 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     6541 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     6601 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     6661 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     6721 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     6781 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     6841 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     6901 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     6961 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg
     7021 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     7081 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     7141 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     7201 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     7261 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     7321 cagggccggc cggcatggcg agtgtgtggg attacgacct ggtactgatg gcggtttccc
     7381 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     7441 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     7501 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     7561 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     7621 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     7681 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     7741 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     7801 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     7861 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     7921 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     7981 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     8041 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
     8101 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     8161 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     8221 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     8281 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     8341 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     8401 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     8461 gcccacatca aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa accttgcgct
     8521 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
     8581 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     8641 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     8701 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     8761 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     8821 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     8881 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     8941 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     9001 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     9061 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     9121 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     9181 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     9241 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     9301 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     9361 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     9421 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     9481 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     9541 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     9601 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     9661 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     9721 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     9781 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa
     9841 ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata gcttgcttgg
     9901 tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca aatactttgc gatcgtgttg
     9961 cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact gcttctctcg caacgccatc
    10021 cgacggatga tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt
    10081 tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa ccggctcagt
    10141 tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg cggcaggata
    10201 tattcaattg taaatggctt catgtccggg aaatctacat ggatcagcaa tgagtatgat
    10261 ggtcaatatg gagaaaaaga aagagtaatt accaattttt tttcaattca aaaatgtaga
    10321 tgtccgcagc gttattataa aatgaaagta cattttgata aaacgacaaa ttacgatccg
    10381 tcgtatttat aggcgaaagc aataaacaaa ttattctaat tcggaaatct ttatttcgac
    10441 gtgtctacat tcacgtccaa atgggggctt agatgagaaa cttcacgatc ggc
//
