LOCUS p6int-Ubi 10682 bp DNA circular SYN 09-JUN-2008 DEFINITION Ligation of Ubi into p6int* ACCESSION p6int-Ubi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10682) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Restriction of pUbi-ABM with Spe-Sal and cloning of the Ubi-promotor into the Xba-Xho sites of p6int. Exchange of promotor. FEATURES Location/Qualifiers misc_feature 10..1517 /gene="Ubi-int" /product="Promotor" /SECDrawAs="Region" misc_feature 997..1516 /gene="Intron" /product="Intron" /SECDrawAs="Region" CDS 1544..1961 /gene="hpt" /product="Hygromycin" /SECDrawAs="Gene" misc_feature 1962..2150 /gene="STLS1" /product="Intron STLS1" /SECDrawAs="Region" CDS 2151..2758 /gene="hpt" /product="Hygromycin" /SECDrawAs="Gene" misc_feature 2783..2984 /gene="T35S" /product="Terminator 35S" /SECDrawAs="Region" misc_feature 3157..3180 /gene="BR" /product="right border" /SECDrawAs="Region" misc_feature 3715..4412 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" misc_feature 4961..8666 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" CDS 8850..9860 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" misc_feature 10381..10404 /gene="BL" /product="left border" /SECDrawAs="Region" ORIGIN 1 tctagtagtc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt 61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca 121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta 181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag 241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt 301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat 361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta 421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata 481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga 541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa 601 cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc 661 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc 721 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga 781 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga 841 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc 901 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc 961 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc 1021 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta 1081 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt 1141 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt 1201 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag gataggtata 1261 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata 1321 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga 1381 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc 1441 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt 1501 ggtgttactt ctgcaggtcg agggccccgg ggggcaataa gatatgaaaa agcctgaact 1561 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat 1621 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata 1681 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca 1741 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag 1801 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac 1861 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga 1921 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggtaagtttc tgcttctacc 1981 tttgatatat atataataat tatcattaat tagtagtaat ataatatttc aaatattttt 2041 ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata 2101 ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag gtatcggtca 2161 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 2221 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 2281 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 2341 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 2401 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 2461 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 2521 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 2581 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 2641 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 2701 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag 2761 tagatgccga ccggagtccg caaaaatcac cagtctctct ctacaaatct atctctctct 2821 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg 2881 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata 2941 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcaa 3001 gctgatccac tagaggccat ggcggccgca ctagtggatc ccccgggatg catgaattca 3061 agcttatcga tgtcgacagg ccttaagggc cagatcttgg gcccggtacc cgatcagatt 3121 gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac 3181 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta 3241 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgcttggca 3301 ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg acgacggagc 3361 agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag tgggctgctg 3421 tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca 3481 aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca 3541 gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg gatctcgcct 3601 tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg 3661 tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgaaaagg 3721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 4141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 4201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 4261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4501 gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta 4621 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg 4681 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc 4741 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 4801 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 4861 accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc tgctatagtg 4921 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct 4981 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta 5041 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg 5101 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc 5161 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca 5221 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg 5281 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg 5341 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca 5401 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt 5461 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga ggcgtgaagt 5521 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc 5581 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt 5641 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct 5701 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag 5761 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga 5821 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac 5881 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg cctggccggc 5941 gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta 6001 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg 6061 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg 6121 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc 6181 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta 6241 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc 6301 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag 6361 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc 6421 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc 6481 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg 6541 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc 6601 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag 6661 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga 6721 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg 6781 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc 6841 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg 6901 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt 6961 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac 7021 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt 7081 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc 7141 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt 7201 tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat 7261 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg 7321 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc 7381 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt 7441 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag 7501 aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg gtactgatgg 7561 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg 7621 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg 7681 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca 7741 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct 7801 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg 7861 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg 7921 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac 7981 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg 8041 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg 8101 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc 8161 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc 8221 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca 8281 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa 8341 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg 8401 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg 8461 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc 8521 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa 8581 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg 8641 accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg gagaccgaaa 8701 ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg 8761 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt 8821 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga 8881 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt 8941 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat 9001 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt 9061 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg 9121 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag 9181 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc 9241 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg 9301 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc 9361 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc 9421 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag 9481 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc 9541 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg 9601 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag 9661 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg 9721 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc 9781 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca 9841 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc 9901 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt 9961 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat 10021 atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatag 10081 cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg 10141 atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc 10201 aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc 10261 tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac 10321 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc 10381 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 10441 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 10501 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 10561 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 10621 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 10681 gc //