LOCUS       pLH6500                 9393 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  AY234329 Binary vector pLH6500, complete sequence.
ACCESSION   pLH6500
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|GenBank               9393 bp    DNA     circular SYN 10-
            APR-2003
            ACCESSION   AY234329
            VERSION     AY234329.1  GI:29569718
            KEYWORDS:   . 
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /SECDrawAs="Region"
     CAAT_signal     360..363
                     /gene="CAAT_signal"
                     /SECDrawAs="Info only"
     TATA_signal     392..398
                     /gene="TATA_signal"
                     /SECDrawAs="Info only"
     CDS             452..1477
                     /gene="hph"
                     /product="hygromycin phosphotransferase"
                     /SECDrawAs="Gene"
     misc_feature    1502..1703
                     /gene="T35S"
                     /SECDrawAs="Region"
     polyA_signal    1668..1683
                     /gene="polyA_signal"
                     /SECDrawAs="Info only"
     misc_feature    1868..1891
                     /gene="RB"
                     /SECDrawAs="Region"
     misc_feature    2426..3123
                     /gene="ColE1"
                     /SECDrawAs="Region"
     -35_signal      3737..3742
                     /gene="-35_signal"
                     /SECDrawAs="Info only"
     -10_signal      3763..3768
                     /gene="-10_signal"
                     /SECDrawAs="Info only"
     CDS             3799..4485
                     /gene="resolvase"
                     /product="resolvase"
                     /SECDrawAs="Gene"
     -35_signal      4719..4724
                     /gene="-35_signal"
                     /SECDrawAs="Info only"
     -10_signal      4740..4745
                     /gene="-10_signal"
                     /SECDrawAs="Info only"
     CDS             4784..5413
                     /gene="ParA"
                     /product="ParA"
                     /SECDrawAs="Gene"
     CDS             5740..6915
                     /gene="ParB"
                     /product="ParB"
                     /SECDrawAs="Gene"
     -35_signal      7516..7521
                     /gene="-35_signal"
                     /SECDrawAs="Info only"
     -10_signal      7539..7544
                     /gene="-10_signal"
                     /SECDrawAs="Info only"
     CDS             7561..8571
                     /gene="aadA"
                     /product="streptomycin/spectinomycin adenyltransferase"
                     /SECDrawAs="Gene"
     misc_feature    9092..9112
                     /gene="LB"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag ggccccgggg ggcaataaga tatgaaaaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      901 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      961 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1021 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1081 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1141 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1201 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1261 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1321 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1381 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1441 ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac cggagtccgc
     1501 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     1561 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     1621 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     1681 attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctagaggccc ttaaggcctt
     1741 actagtggat cccccgggat gcatgaattc aagcttatcg atgtcgacgc ggccgccatg
     1801 gccagatctg ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc
     1861 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     1921 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     1981 accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc
     2041 acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc
     2101 tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac
     2161 gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg
     2221 cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg
     2281 atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac
     2341 gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc
     2401 gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct
     2461 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2521 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2581 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2641 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2701 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2761 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2821 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2881 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2941 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3001 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3061 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3181 catgttcttt cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga
     3241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3301 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3361 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3421 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg
     3601 aggtcgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     3661 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     3721 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     3781 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     3841 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     3901 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     3961 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     4021 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     4081 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     4141 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     4201 cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc gccctaccct caccccggca
     4261 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     4321 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     4381 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     4441 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     4501 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     4561 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     4621 tgtctgatgc caagctcgcg gcctggccgg cgagcttggc cgctgaagaa accgagcgcc
     4681 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     4741 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     4801 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     4861 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     4921 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     4981 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     5041 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     5101 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     5161 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     5221 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     5281 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     5341 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     5401 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     5461 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     5521 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     5581 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     5641 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     5701 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     5761 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     5821 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     5881 gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa
     5941 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     6001 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     6061 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     6121 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     6181 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcg
     6241 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     6301 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     6361 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     6421 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     6481 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     6541 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     6601 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     6661 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     6721 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     6781 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     6841 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     6901 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     6961 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     7021 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     7081 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     7141 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     7201 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     7261 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     7321 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccggt
     7381 gggtatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga
     7441 aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     7501 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     7561 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     7621 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     7681 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     7741 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     7801 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     7861 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     7921 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     7981 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     8041 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     8101 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     8161 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     8221 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     8281 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     8341 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     8401 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     8461 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     8521 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca
     8581 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     8641 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
     8701 cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt ttcttgttat
     8761 cgcaatagtt ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg tgaacgtcgg
     8821 ctcgattgta cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc cggtgcgtcg
     8881 gctgatctca cggatcgact gcttctctcg caacgccatc cgacggatga tgtttaaaag
     8941 tcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt tggggggaag gtgcacatgg
     9001 ctcagttctc aatggaaatt atctgcctaa ccggctcagt tctgcgtaga aaccaacatg
     9061 caagctccac cgggtgcaaa gcggcagcgg cggcaggata tattcaattg taaatggctt
     9121 catgtccggg aaatctacat ggatcagcaa tgagtatgat ggtcaatatg gagaaaaaga
     9181 aagagtaatt accaattttt tttcaattca aaaatgtaga tgtccgcagc gttattataa
     9241 aatgaaagta cattttgata aaacgacaaa ttacgatccg tcgtatttat aggcgaaagc
     9301 aataaacaaa ttattctaat tcggaaatct ttatttcgac gtgtctacat tcacgtccaa
     9361 atgggggctt agatgagaaa cttcacgatc ggc
//
