LOCUS       p9-35S-OCS              9782 bp    DNA     circular SYN 20-AUG-2008
DEFINITION  Ligation of 35S-OCS into pLH9000*
ACCESSION   p9-35S-OCS
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     CDS             439..1242
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
     misc_feature    1255..1456
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1508..1925
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     CDS             1896..1902
                     /gene="(null)"
                     /product="TATA-box"
                     /SECDrawAs="Gene"
     misc_feature    1977..2172
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
     CDS             complement (1998..2017)
                     /gene="OR"
                     /product="Primer OR"
                     /SECDrawAs="Gene"
     misc_feature    2257..2280
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
     misc_feature    2815..3512
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4061..7766
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     misc_feature    7950..8960
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Region"
     misc_feature    9481..9504
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      481 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      541 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      601 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      661 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      721 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      781 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      841 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      901 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      961 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     1021 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     1081 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1141 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1201 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1261 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1321 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1381 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1441 aaaccaaaat ccagtgacct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     1501 cactagatca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     1561 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     1621 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat
     1681 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc
     1741 gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt
     1801 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     1861 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     1921 gagaggacac tagttggatc ccccgggctg caggaattca agcttacgcg tgtcgaccct
     1981 gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt
     2041 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
     2101 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
     2161 tactgattgt cctcgacagg ccttaagggc cagatcttgg gcccggtacc cgatcagatt
     2221 gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     2281 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     2341 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgcttggca
     2401 ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg acgacggagc
     2461 agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag tgggctgctg
     2521 tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca
     2581 aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca
     2641 gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg gatctcgcct
     2701 tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg
     2761 tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgaaaagg
     2821 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3541 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3601 gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3661 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3721 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3781 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3841 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3901 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3961 accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc tgctatagtg
     4021 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     4081 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     4141 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     4201 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     4261 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     4321 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     4381 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     4441 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     4501 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     4561 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga ggcgtgaagt
     4621 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     4681 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     4741 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     4801 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     4861 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     4921 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     4981 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg cctggccggc
     5041 gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     5101 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     5161 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     5221 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     5281 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     5341 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     5401 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     5461 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     5521 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     5581 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     5641 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     5701 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     5761 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     5821 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     5881 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     5941 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     6001 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     6061 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     6121 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     6181 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     6241 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     6301 tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     6361 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     6421 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     6481 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     6541 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     6601 aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg gtactgatgg
     6661 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     6721 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     6781 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     6841 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     6901 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     6961 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     7021 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     7081 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     7141 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     7201 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     7261 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     7321 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     7381 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     7441 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     7501 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     7561 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     7621 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     7681 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     7741 accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg gagaccgaaa
     7801 ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg
     7861 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     7921 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     7981 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     8041 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     8101 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     8161 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     8221 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     8281 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     8341 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     8401 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     8461 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     8521 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     8581 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     8641 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     8701 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     8761 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     8821 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     8881 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     8941 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     9001 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     9061 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     9121 atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatag
     9181 cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg
     9241 atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc
     9301 aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc
     9361 tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac
     9421 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc
     9481 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
     9541 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
     9601 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
     9661 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
     9721 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
     9781 gc
//
