LOCUS       p95P-Nos                9026 bp    DNA     circular SYN 26-JUL-2005
DEFINITION  Ligation of 95P-Nos* into pLH9500*
ACCESSION   p95P-Nos
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    7..304
                     /gene="P-NOS"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    319..1122
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    1135..1336
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1554
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Marker"
     misc_feature    2059..2756
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3305..7010
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     misc_feature    7194..8204
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Region"
     misc_feature    8738
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Marker"
ORIGIN      
        1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
       61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      301 gcacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1141 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1201 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1261 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1321 aaaccaaaat ccagtgacct gcaggcatgc aagctagagg cccttaaggc cttactagtg
     1381 gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga cgcggccgcc atggccagat
     1441 ctgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt
     1501 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     1561 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     1621 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa
     1681 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg
     1741 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc
     1801 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg
     1861 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct
     1921 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata
     1981 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg
     2041 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2821 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata
     2881 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2941 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     3001 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3061 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3121 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3181 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga
     3241 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     3301 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     3361 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     3421 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     3481 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     3541 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     3601 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     3661 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     3721 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     3781 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     3841 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     3901 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     3961 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     4021 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     4081 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     4141 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     4201 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     4261 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct
     4321 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     4381 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     4441 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     4501 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     4561 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     4621 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     4681 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     4741 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     4801 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     4861 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     4921 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     4981 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     5041 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     5101 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     5161 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     5221 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     5281 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     5341 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     5401 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
     5461 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     5521 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg
     5581 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     5641 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     5701 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     5761 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     5821 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt
     5881 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     5941 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     6001 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     6061 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     6121 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     6181 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     6241 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     6301 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     6361 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     6421 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     6481 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     6541 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     6601 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     6661 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     6721 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     6781 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     6841 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     6901 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     6961 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg
     7021 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct
     7081 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     7141 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     7201 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     7261 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     7321 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     7381 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     7441 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     7501 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     7561 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     7621 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     7681 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     7741 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     7801 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     7861 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     7921 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     7981 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     8041 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     8101 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     8161 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt
     8221 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     8281 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     8341 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata
     8401 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt
     8461 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc
     8521 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg
     8581 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt
     8641 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc
     8701 caccgggtgc aaagcggcag cggcggcagg atatattcaa ttgtaaatgg cttcatgtcc
     8761 gggaaatcta catggatcag caatgagtat gatggtcaat atggagaaaa agaaagagta
     8821 attaccaatt ttttttcaat tcaaaaatgt agatgtccgc agcgttatta taaaatgaaa
     8881 gtacattttg ataaaacgac aaattacgat ccgtcgtatt tataggcgaa agcaataaac
     8941 aaattattct aattcggaaa tctttatttc gacgtgtcta cattcacgtc caaatggggg
     9001 cttagatgag aaacttcacg atcggc
//
