LOCUS       4.p95U10               10211 bp    DNA     circular SYN 14-MAY-2009
DEFINITION  Ligation of UbiAT-Spe-Xho* into pLH9500*
ACCESSION   4.p95U10
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pLH9500*
               Fragment ends: XhoI and XbaI
               Fragment size: 8722
            Insert molecule:  UbiAT-Spe-Xho*
               Fragment ends: SpeI and XhoI
               Fragment size: 1489
FEATURES             Location/Qualifiers
     misc_feature    7..1484
                     /gene="UBQ10"
                     /product="Promotor Ubi A.thaliana"
                     /SECDrawAs="Region"
     misc_feature    1113..1178
                     /gene="(null)"
                     /product="Ubi cds 5`"
                     /SECDrawAs="Region"
     misc_feature    1179..1482
                     /gene="íntron"
                     /SECDrawAs="Region"
     CDS             1504..2307
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
     misc_feature    2320..2521
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    2686..2709
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
     misc_feature    3244..3941
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    4490..8195
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             8379..9389
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9910..9933
                     /gene="BL"
                     /product="Border-left"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
       61 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      121 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      181 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      241 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      301 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      361 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      421 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      481 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      541 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      601 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      661 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
      721 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
      781 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
      841 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
      901 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
      961 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1021 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1081 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1141 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1201 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1261 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1321 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1381 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1441 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agatactagc tcgagtggcc
     1501 accatgggag cttggattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     1561 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     1621 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     1681 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     1741 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     1801 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     1861 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     1921 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     1981 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     2041 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     2101 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     2161 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     2221 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     2281 tatcgccttc ttgacgagtt cttctgagcg ggacccgcaa aaatcaccag tctctctcta
     2341 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     2401 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     2461 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     2521 gacctgcagg catgcaagct agaggccctt aaggccttac tagtggatcc cccgggatgc
     2581 atgaattcaa gcttatcgat gtcgacgcgg ccgccatggc cagatctggg cccggtaccc
     2641 gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg
     2701 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     2761 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag
     2821 tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga
     2881 cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt
     2941 gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc
     3001 tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg
     3061 tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg
     3121 atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag
     3181 atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg
     3241 ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3301 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3361 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3421 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3481 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3541 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3601 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3661 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3721 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4021 cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     4141 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     4201 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     4261 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4321 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4381 accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct
     4441 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     4501 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     4561 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     4621 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     4681 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     4741 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     4801 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     4861 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     4921 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     4981 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag
     5041 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     5101 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     5161 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     5221 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     5281 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     5341 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     5401 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc
     5461 ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     5521 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     5581 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     5641 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     5701 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     5761 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     5821 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     5881 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     5941 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     6001 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     6061 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     6121 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     6181 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     6241 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     6301 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     6361 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     6421 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     6481 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     6541 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     6601 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     6661 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     6721 tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     6781 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     6841 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     6901 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     6961 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     7021 ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg
     7081 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     7141 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     7201 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     7261 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     7321 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     7381 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     7441 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     7501 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     7561 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     7621 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     7681 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     7741 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     7801 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     7861 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     7921 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     7981 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     8041 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     8101 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     8161 cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg
     8221 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc
     8281 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     8341 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     8401 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     8461 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     8521 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     8581 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     8641 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     8701 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     8761 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     8821 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     8881 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     8941 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     9001 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     9061 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     9121 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     9181 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     9241 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     9301 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     9361 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     9421 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     9481 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     9541 gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc
     9601 gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa
     9661 tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc
     9721 ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg
     9781 ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat
     9841 ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc
     9901 ggcagcggcg gcaggatata ttcaattgta aatggcttca tgtccgggaa atctacatgg
     9961 atcagcaatg agtatgatgg tcaatatgga gaaaaagaaa gagtaattac caattttttt
    10021 tcaattcaaa aatgtagatg tccgcagcgt tattataaaa tgaaagtaca ttttgataaa
    10081 acgacaaatt acgatccgtc gtatttatag gcgaaagcaa taaacaaatt attctaattc
    10141 ggaaatcttt atttcgacgt gtctacattc acgtccaaat gggggcttag atgagaaact
    10201 tcacgatcgg c
//
