LOCUS       p9L                     9224 bp    DNA     circular SYN 21-SEP-2007
DEFINITION  Ligation of bar-int-Lox-Xba-Sbf* into p7int*
ACCESSION   p9L
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p7int*
               Fragment ends: SbfI and XbaI
               Fragment size: 7692
            Insert molecule:  bar-int-Lox-Xba-Sbf*
               Fragment ends: XbaI and SbfI
               Fragment size: 1478
FEATURES             Location/Qualifiers
     misc_feature    7..40
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Region"
     misc_feature    41..456
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     CDS             473..1276
                     /gene="NPTII"
                     /SECDrawAs="Gene"
     misc_feature    1289..1490
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1494..1527
                     /gene="Lox"
                     /product="LoxP511"
                     /SECDrawAs="Region"
     misc_feature    1699..1722
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
     misc_feature    2257..2954
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3503..7208
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             7392..8402
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    8923..8946
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaataa cttcgtataa tgtatactat acgaagttat acatggtgga gcacgacact
       61 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact
      121 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      181 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa
      241 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc
      301 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      361 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      421 tcctctatat aaggaagttc atttcatttg gagaggacct cgagtggcca ccatgggagc
      481 ttggattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
      541 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
      601 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
      661 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
      721 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
      781 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
      841 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
      901 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
      961 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1021 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1081 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1141 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1201 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1261 tgacgagttc ttctgagcgg gacccgcaaa aatcaccagt ctctctctac aaatctatct
     1321 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     1381 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1441 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg accataactt
     1501 cgtataatgt atactatacg aagttatcct gcaggcatgc aagctgatcc actagaggcc
     1561 atggcggccg cactagtgga tcccccggga tgcatgaatt caagcttatc gatgtcgaca
     1621 ggccttaagg gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg
     1681 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     1741 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     1801 tgtgcatgcc aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact
     1861 tctgttcaac cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc
     1921 cttggctcgt ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg
     1981 cggtcgcgga cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa
     2041 agcgtgatct gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt
     2101 tccgtcacag gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc
     2161 gggaactcca cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct
     2221 gggcagtcgc cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt
     2281 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2341 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2401 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2461 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2521 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2581 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2641 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2701 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2761 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2821 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2881 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2941 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3001 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg
     3061 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3121 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3181 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     3241 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     3301 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3361 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     3421 ggggtacgtc gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     3481 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     3541 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     3601 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     3661 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     3721 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     3781 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     3841 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     3901 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     3961 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     4021 cccggagcgg gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc
     4081 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     4141 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     4201 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     4261 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     4321 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     4381 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat
     4441 cctggccggt ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga
     4501 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     4561 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     4621 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     4681 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     4741 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     4801 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     4861 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     4921 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     4981 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     5041 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     5101 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     5161 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     5221 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     5281 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     5341 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     5401 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     5461 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     5521 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     5581 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat
     5641 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
     5701 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg
     5761 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     5821 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     5881 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     5941 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     6001 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     6061 ccggcatggc gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     6121 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     6181 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     6241 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     6301 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     6361 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     6421 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     6481 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     6541 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     6601 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     6661 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     6721 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     6781 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     6841 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     6901 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     6961 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     7021 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     7081 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     7141 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     7201 aaggcaccgg tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca
     7261 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
     7321 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     7381 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     7441 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     7501 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     7561 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
     7621 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7681 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7741 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     7801 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     7861 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     7921 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     7981 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     8041 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     8101 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     8161 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     8221 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     8281 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     8341 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     8401 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     8461 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     8521 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt
     8581 tttcttgtta tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc
     8641 gtgaacgtcg gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc
     8701 ccggtgcgtc ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg
     8761 atgtttaaaa gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa
     8821 ggtgcacatg gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag
     8881 aaaccaacat gcaagctcca ccgggtgcaa agcggcagcg gcggcaggat atattcaatt
     8941 gtaaatggct tcatgtccgg gaaatctaca tggatcagca atgagtatga tggtcaatat
     9001 ggagaaaaag aaagagtaat taccaatttt ttttcaattc aaaaatgtag atgtccgcag
     9061 cgttattata aaatgaaagt acattttgat aaaacgacaa attacgatcc gtcgtattta
     9121 taggcgaaag caataaacaa attattctaa ttcggaaatc tttatttcga cgtgtctaca
     9181 ttcacgtcca aatgggggct tagatgagaa acttcacgat cggc
//
