LOCUS       p9N-35S                 9662 bp    DNA     circular SYN 09-NOV-2006
DEFINITION  Ligation of p35OCS-BM* into p9N*
ACCESSION   p9N-35S
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  p9N*
               Fragment ends: SfiI
               Fragment size: 8970
            Insert molecule:  p35OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
            XbaI is normally methylated
FEATURES             Location/Qualifiers
     misc_feature    7..304
                     /gene="P-NOS"
                     /product="Promotor"
                     /SECDrawAs="Region"
     misc_feature    319..1122
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Region"
     misc_feature    1135..1336
                     /gene="T35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1388..1805
                     /gene="P-35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
     CDS             1776..1782
                     /gene="(null)"
                     /product="TATA-box"
                     /SECDrawAs="Gene"
     misc_feature    1857..2052
                     /gene="T-OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
     misc_feature    2137..2160
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
     misc_feature    2695..3392
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3429..3688
                     /gene="BOM"
                     /product="Bom-site"
                     /SECDrawAs="Region"
     misc_feature    3941..7646
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     misc_feature    7830..8840
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Region"
     misc_feature    9361..9384
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
       61 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      121 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      181 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      241 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      301 gcacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggttct
      361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggacc cgcaaaaatc
     1141 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     1201 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     1261 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     1321 aaaccaaaat ccagtgacct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     1381 cactagatca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     1441 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     1501 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat
     1561 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc
     1621 gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt
     1681 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     1741 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     1801 gagaggacac tagttggatc ccccgggctg caggaattca agcttacgcg tgtcgaccct
     1861 gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt
     1921 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
     1981 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
     2041 tactgattgt cctcgacagg ccttaagggc cagatcttgg gcccggtacc cgatcagatt
     2101 gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     2161 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     2221 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgcttggca
     2281 ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg acgacggagc
     2341 agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag tgggctgctg
     2401 tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca
     2461 aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca
     2521 gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg gatctcgcct
     2581 tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg
     2641 tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgaaaagg
     2701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3481 gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3601 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3661 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3721 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3781 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3841 accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc tgctatagtg
     3901 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     3961 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     4021 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     4081 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     4141 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     4201 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     4261 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     4321 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     4381 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     4441 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga ggcgtgaagt
     4501 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     4561 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     4621 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     4681 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     4741 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     4801 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     4861 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg cctggccggc
     4921 gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     4981 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     5041 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     5101 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     5161 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     5221 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     5281 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     5341 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     5401 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     5461 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     5521 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     5581 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     5641 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     5701 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     5761 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     5821 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     5881 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     5941 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     6001 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     6061 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     6121 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     6181 tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     6241 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     6301 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     6361 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     6421 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     6481 aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg gtactgatgg
     6541 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     6601 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     6661 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     6721 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     6781 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     6841 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     6901 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     6961 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     7021 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     7081 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     7141 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     7201 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     7261 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     7321 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     7381 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     7441 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     7501 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     7561 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     7621 accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg gagaccgaaa
     7681 ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg
     7741 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     7801 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     7861 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     7921 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     7981 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     8041 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     8101 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     8161 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     8221 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     8281 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     8341 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     8401 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     8461 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     8521 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     8581 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     8641 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     8701 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     8761 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     8821 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     8881 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     8941 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     9001 atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatag
     9061 cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg
     9121 atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc
     9181 aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc
     9241 tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac
     9301 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc
     9361 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
     9421 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
     9481 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
     9541 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
     9601 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
     9661 gc
//
