LOCUS       p9int                   9343 bp    DNA     circular SYN 06-JUN-2002
DEFINITION  Ligation of Npt-int* into pLH9000*
ACCESSION   p9int
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    7..422
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
     CDS             439..475
                     /gene="NptII"
                     /product="Kanamycin resistance"
                     /SECDrawAs="Gene"
     CDS             468..490
                     /gene="NOL1F"
                     /product="Primer NOL1F"
                     /SECDrawAs="Gene"
     misc_feature    476..664
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Region"
     CDS             complement (463..490)
                     /gene="Nol1R"
                     /product="Primer NOL1R"
                     /SECDrawAs="Gene"
     CDS             653..675
                     /gene="NOL2F"
                     /product="Primer NOL2F"
                     /SECDrawAs="Gene"
     CDS             665..1431
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
     CDS             complement (653..673)
                     /gene="Nol2R"
                     /product="Primer Nol2R"
                     /SECDrawAs="Gene"
     misc_feature    1444..1645
                     /gene="T-35S"
                     /product="Terminator 35S"
                     /SECDrawAs="Region"
     misc_feature    1818..1841
                     /gene="BR"
                     /product="Border-right"
                     /SECDrawAs="Region"
     misc_feature    2376..3073
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
     misc_feature    3622..7327
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
     CDS             7511..8521
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
     misc_feature    9042..9065
                     /gene="BL"
                     /product="Border-Left"
                     /SECDrawAs="Region"
ORIGIN      
        1 tctagaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
       61 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      121 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
      181 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
      241 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
      301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      421 ggacctcgag tggccaccat gggagcttgg attgaacaag atggattgca cgcaggtaag
      481 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata
      541 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt
      601 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt
      661 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      721 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      781 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      841 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      901 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      961 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     1021 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     1081 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     1141 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     1201 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     1261 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1321 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1381 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggaccc
     1441 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     1501 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     1561 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     1621 taattcctaa aaccaaaatc cagtgacctg caggcatgca agctgatcca ctagaggcca
     1681 tggcggccgc actagtggat cccccgggat gcatgaattc aagcttatcg atgtcgacag
     1741 gccttaaggg ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt
     1801 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     1861 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     1921 gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt
     1981 ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc
     2041 ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc
     2101 ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa
     2161 gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt
     2221 ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg
     2281 ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg
     2341 ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg aagatccttt
     2401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgattaccgc
     3181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3241 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3301 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3361 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3481 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     3541 gggtacgtcg aggtcgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     3601 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     3661 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     3721 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     3781 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     3841 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     3901 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     3961 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     4021 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     4081 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     4141 ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc gccctaccct
     4201 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     4261 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     4321 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     4381 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     4441 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     4501 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     4561 ctggccggtt tgtctgatgc caagctcgcg gcctggccgg cgagcttggc cgctgaagaa
     4621 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     4681 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     4741 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     4801 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     4861 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     4921 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     4981 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     5041 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     5101 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     5161 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     5221 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     5281 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     5341 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     5401 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     5461 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     5521 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     5581 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     5641 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     5701 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     5761 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     5821 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc
     5881 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     5941 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     6001 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     6061 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     6121 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     6181 cggcatggcg agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     6241 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     6301 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     6361 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     6421 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     6481 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     6541 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     6601 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     6661 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     6721 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     6781 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     6841 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     6901 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     6961 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     7021 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     7081 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     7141 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     7201 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     7261 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     7321 aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag
     7381 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     7441 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     7501 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     7561 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     7621 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     7681 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     7741 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     7801 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     7861 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     7921 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     7981 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     8041 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     8101 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     8161 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     8221 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     8281 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     8341 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     8401 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     8461 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     8521 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     8581 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8641 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     8701 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg
     8761 tgaacgtcgg ctcgattgta cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc
     8821 cggtgcgtcg gctgatctca cggatcgact gcttctctcg caacgccatc cgacggatga
     8881 tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt tggggggaag
     8941 gtgcacatgg ctcagttctc aatggaaatt atctgcctaa ccggctcagt tctgcgtaga
     9001 aaccaacatg caagctccac cgggtgcaaa gcggcagcgg cggcaggata tattcaattg
     9061 taaatggctt catgtccggg aaatctacat ggatcagcaa tgagtatgat ggtcaatatg
     9121 gagaaaaaga aagagtaatt accaattttt tttcaattca aaaatgtaga tgtccgcagc
     9181 gttattataa aatgaaagta cattttgata aaacgacaaa ttacgatccg tcgtatttat
     9241 aggcgaaagc aataaacaaa ttattctaat tcggaaatct ttatttcgac gtgtctacat
     9301 tcacgtccaa atgggggctt agatgagaaa cttcacgatc ggc
//
