LOCUS       pGFP-oI                 5568 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  pGFP-oI Circularized
ACCESSION   pGFP-oI
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    91..124
                     /gene="loxP"
                     /product="LoxP511"
     misc_feature    140..1648
                     /gene="Ubi-int"
                     /product="Promotor"
     misc_feature    1127..1646
                     /gene="Intron"
                     /product="Intron"
     CDS             1721..2437
                     /gene="GFP"
     misc_feature    2444..2697
                     /gene="NOS"
                     /product="NOS-Terminator"
     misc_feature    2704..2737
                     /gene="LoxP"
                     /product="LoxP511"
     misc_feature    complement (3223..3939)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (3982..4842)
                     /gene="Amp"
                     /product="ORF-1"
ORIGIN      
        1 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg
       61 cccaagatct ggcccttagg gccttaatta ataacttcgt ataatgtata ctatacgaag
      121 ttattgcggc cgcactaggc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat
      181 aatgagcatt gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt
      241 ttgaagtgca gtttatctat ctttatacat atatttaaac tttactctac gaataatata
      301 atctatagta ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca
      361 tggtctaaag gacaattgag tattttgaca acaggactct acagttttat ctttttagtg
      421 tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt
      481 attagtacat ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac
      541 atctatttta ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt
      601 ttatttaata atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata
      661 ccctttaaga aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca
      721 gcctgttaaa cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
      781 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
      841 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
      901 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
      961 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
     1021 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
     1081 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
     1141 ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc
     1201 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc
     1261 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag
     1321 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag
     1381 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1441 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1501 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1561 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1621 cctgttgttt ggtgttactt ctgcaggtac tagtgggtac cagagctccc taggttctag
     1681 aaccggtgac gtctcccatg gtgaagcttg gatccggccg atgggcaagg gcgaggaact
     1741 gttcactggc gtggtcccaa tcctggtgga actggatggt gatgtgaacg ggcacaagtt
     1801 ctccgtcagc ggagagggtg aaggtgatgc cacctacgga aagctcaccc tgaagttcat
     1861 ctgcactacc ggaaagctcc ctgttccgtg gccaaccctc gtcaccactt tcacctacgg
     1921 tgttcagtgc ttctcccggt acccagatca catgaagcag catgacttct tcaagagcgc
     1981 catgcccgaa ggctacgtgc aagaaaggac tatcttcttc aaggatgacg ggaactacaa
     2041 gacacgtgcc gaagtcaagt tcgaaggtga taccctggtg aaccgcatcg agctgaaagg
     2101 tatcgatttc aaggaagatg gaaacatcct cggacacaag ctggagtaca actacaactc
     2161 ccacaacgta tacatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2221 caggcacaac atcgaagatg gaagcgtgca actggcggac cactaccagc agaacacgcc
     2281 catcggcgat ggccctgtcc tgctgccgga caaccattac ctgtccacgc aatctgccct
     2341 ctccaaggac cccaacgaga agagggacca catggtcctg ctggagttcg tgacggctgc
     2401 tgggatcacg catggcatgg atgaactcta caagtgactc gacgatcgtt caaacatttg
     2461 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     2521 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     2581 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     2641 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggta
     2701 cctataactt cgtataatgt atactatacg aagttattta aattggcccc catggcctct
     2761 agagagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
     2821 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2881 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2941 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3001 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     4981 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5041 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5101 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5161 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5221 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5281 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5341 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5401 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     5461 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5521 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgt
//
