LOCUS       pLNU-GFP                5758 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  Ligation of L29GFPeag* into pLNU-BA*
ACCESSION   pLNU-GFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    91..124
                     /gene="loxP"
                     /product="LoxP511"
     misc_feature    140..1648
                     /gene="Ubi-int"
                     /product="Promotor"
     misc_feature    1127..1646
                     /gene="Intron"
                     /product="Intron"
     misc_feature    1698..1700
                     /gene="(null)"
                     /product="Start in MCS"
     CDS             1721..2099
                     /gene="GFP"
                     /product="GFP"
     CDS             2100..2288
                     /gene="ST-LS1"
                     /product="Intron"
     CDS             2289..2626
                     /gene="GFP"
                     /product="GFP"
     misc_feature    2633..2886
                     /gene="Tnos"
                     /product="NOS-Terminator"
     misc_feature    2893..2926
                     /gene="LoxP"
                     /product="LoxP511"
     misc_feature    complement (3412..4128)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (4171..5031)
                     /gene="Amp"
                     /product="ORF-1"
ORIGIN      
        1 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg
       61 cccaagatct ggcccttagg gccttaatta ataacttcgt ataatgtata ctatacgaag
      121 ttattgcggc cgcactaggc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat
      181 aatgagcatt gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt
      241 ttgaagtgca gtttatctat ctttatacat atatttaaac tttactctac gaataatata
      301 atctatagta ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca
      361 tggtctaaag gacaattgag tattttgaca acaggactct acagttttat ctttttagtg
      421 tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt
      481 attagtacat ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac
      541 atctatttta ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt
      601 ttatttaata atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata
      661 ccctttaaga aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca
      721 gcctgttaaa cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
      781 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
      841 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
      901 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
      961 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
     1021 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
     1081 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
     1141 ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc
     1201 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc
     1261 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag
     1321 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag
     1381 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1441 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1501 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1561 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1621 cctgttgttt ggtgttactt ctgcaggtac tagtgggtac cagagctccc taggttctag
     1681 aaccggtgac gtctcccatg gtgaagcttg gatccggccg atgggcaagg gcgaggaact
     1741 gttcactggc gtggtcccaa tcctggtgga actggatggt gatgtgaacg ggcacaagtt
     1801 ctccgtcagc ggagagggtg aaggtgatgc cacctacgga aagctcaccc tgaagttcat
     1861 ctgcactacc ggaaagctcc ctgttccgtg gccaaccctc gtcaccactt tcacctacgg
     1921 tgttcagtgc ttctcccggt acccagatca catgaagcag catgacttct tcaagagcgc
     1981 catgcccgaa ggctacgtgc aagaaaggac tatcttcttc aaggatgacg ggaactacaa
     2041 gacacgtgcc gaagtcaagt tcgaaggtga taccctggtg aaccgcatcg agctgaaagg
     2101 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat
     2161 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt
     2221 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg
     2281 atgtgcaggt atcgatttca aggaagatgg aaacatcctc ggacacaagc tggagtacaa
     2341 ctacaactcc cacaacgtat acatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2401 cttcaagatc aggcacaaca tcgaagatgg aagcgtgcaa ctggcggacc actaccagca
     2461 gaacacgccc atcggcgatg gccctgtcct gctgccggac aaccattacc tgtccacgca
     2521 atctgccctc tccaaggacc ccaacgagaa gagggaccac atggtcctgc tggagttcgt
     2581 gacggctgct gggatcacgc atggcatgga tgaactctac aagtgactcg acgatcgttc
     2641 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     2701 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     2761 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     2821 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     2881 agatcggtac ctataacttc gtataatgta tactatacga agttatttaa attggccccc
     2941 atggcctcta gagagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     3001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3841 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5161 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5581 taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg
     5641 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5701 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgt
//
