LOCUS       pLN                     2227 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Cloning of Nos into pLM
ACCESSION   pLN
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    46..79
                     /gene="Lox"
                     /product="LoxP511"
     misc_feature    152..405
                     /gene="NOS-T"
                     /product="NOS-T"
     misc_feature    412..445
                     /gene="Lox"
                     /product="LoxP511"
     misc_feature    complement (495..1161)
                     /gene="ColE1"
                     /product="ColE1"
     CDS             complement (1211..2071)
                     /gene="Amp"
                     /product="Amp-DCS1'"
ORIGIN      
        1 catttccccg aaaagtgcca ctagaggccc ttagggcctt aattaataac ttcgtataat
       61 gtatactata cgaagttatt gcggccgcac tagttggatc ctggccggcc ctgcagggaa
      121 ttccaagctt ggcgcgccga gctcagtcga cgatcgttca aacatttggc aataaagttt
      181 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
      241 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
      301 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
      361 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcggtacc tataacttcg
      421 tataatgtat actatacgaa gttatttaaa ttggccccca aggcctctag taaaaaggcc
      481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1141 taagggattt tggccatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1201 aagaccatga ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1501 gtttgcgcaa cgttgttgcc attgctgctg gcatcgtggt gtcacgctcg tcgtttggta
     1561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1801 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     1861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2041 taagggcgac acggaaatgt tgaatactca tggctgtcag accaagttta ctcatatata
     2101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2161 gataatctca tgggcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2221 ccgcgca
//