LOCUS       pNos-AM                 3231 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Nos-AB-Mlu rotated to position 602
ACCESSION   pNos-AM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    956..1816
                     /gene="Amp"
                     /product="ampecillin resistance"
     CDS             1859..2575
                     /gene="ColE1"
                     /product="ColE1"
     misc_feature    3116..138
                     /gene="NOS-T"
                     /product="Nos-Terminator"
ORIGIN      
        1 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
       61 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
      121 atctatgtta ctagatcgct cgacgcggcc gccatggcca gatctgggcc cggtacccaa
      181 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      241 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      301 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      361 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      421 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      481 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      541 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      601 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      661 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      721 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      781 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
      841 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1081 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1141 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1261 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1321 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1381 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1501 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1561 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1621 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1681 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1741 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1801 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2101 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2701 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2761 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2821 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2881 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     2941 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
     3001 taaccctcac taaagggaac aaaagctgga gctctctaga ggcccttaag gccttactag
     3061 tggatccccc gggctgcagg aattcaagct tacgcgtgtc gactcgaatt tccccgatcg
     3121 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3181 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt a
//