LOCUS       pNos-BM                 3230 bp    DNA     circular SYN 25-JUN-2010
DEFINITION  Nos-BA-M rotated to position 2930
ACCESSION   pNos-BM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    186..439
                     /gene="NOS-T"
                     /product="NOS-T"
     misc_feature    1256..2116
                     /gene="Amp"
                     /product="ORF-1"
     CDS             2159..2875
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggctgcagg aattcaagct tacgcgtgtc gactcgaatt
      181 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
      241 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
      301 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
      361 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
      421 catctatgtt actagatcgc tcgacaggcc ttaagggcca gatcttgggc ccggtaccca
      481 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      541 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      601 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      661 gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      721 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      781 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      841 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      901 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      961 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1021 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1081 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     1141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
//