LOCUS pLN35G-Flpi 6097 bp DNA circular SYN 25-JUN-2010 DEFINITION Ligation of p9-35S-OCS* into pLNUG-FLPint* ACCESSION pLN35G-Flpi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 6097) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software FEATURES Location/Qualifiers misc_feature 91..124 /gene="loxP" /product="LoxP511" misc_feature 140..557 /gene="35S-Pro" /product="Promotor-Region" CDS 591..1273 /gene="Flp" CDS 1274..1462 /gene="int" /product="intron Stls1" CDS 1463..2049 /gene="Flp" CDS 2061..2439 /gene="GFP" /product="GFP" CDS 2440..2628 /gene="int" /product="Intron STLS1" CDS 2629..2966 /gene="GFP" /product="GFP" misc_feature 2973..3226 /gene="NOS" /product="NOS-Terminator" misc_feature 3233..3266 /gene="LoxP" /product="LoxP511" misc_feature complement (3752..4468) /gene="ColE1" /product="ColE1" CDS complement (4511..5371) /gene="Amp" /product="ORF-1" ORIGIN 1 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg 61 cccaagatct ggcccttagg gccttaatta ataacttcgt ataatgtata ctatacgaag 121 ttattgcggc cgcactagat caacatggtg gagcacgaca ctctcgtcta ctccaagaat 181 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata 241 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 301 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa 361 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa 421 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac 481 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 541 tcatttcatt tggagaggac actaggttct agaaccggtg acgtctaagc atgccacaat 601 ttgatatatt atgtaaaaca ccacctaagg tgcttgttcg tcagtttgtg gaaaggtttg 661 aaagaccttc aggtgagaaa atagcattat gtgctgctga actaacctat ttatgttgga 721 tgattacaca taacggaaca gcaatcaaga gagccacatt catgagctat aatactatca 781 taagcaattc gctgagtttc gatattgtca ataaatcact ccagtttaaa tacaagacgc 841 aaaaagcaac aattctggaa gcctcattaa agaaattgat tcctgcttgg gaatttacaa 901 ttattcctta ctatggacaa aaacatcaat ctgatatcac tgatattgta agtagtttgc 961 aattacagtt cgaatcatcg gaagaagcag ataagggaaa tagccacagt aaaaaaatgc 1021 ttaaagcact tctaagtgag ggtgaaagca tctgggagat cactgagaaa atactaaatt 1081 cgtttgagta tacttcgaga tttacaaaaa caaaaacttt ataccaattc ctcttcctag 1141 ctactttcat caattgtgga agattcagcg atattaagaa cgttgatccg aaatcattta 1201 aattagtcca aaataagtat ctgggagtaa taatccagtg tttagtgaca gagacaaaga 1261 caagcgttag caggtaagtt tctgcttcta cctttgatat atatataata attatcatta 1321 attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc 1381 aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg 1441 accaaaattt gttgatgtgc aggcacatat acttctttag cgcaaggggt aggatcgatc 1501 cacttgtata tttggatgaa tttttgagga attctgaacc agtcctaaaa cgagtaaata 1561 ggaccggcaa ttcttcaagc aataaacagg aataccaatt attaaaagat aacttagtca 1621 gatcgtacaa taaagctttg aagaaaaatg cgccttattc aatctttgct ataaaaaatg 1681 gcccaaaatc tcacattgga agacatttga tgacctcatt tctttcaatg aagggcctaa 1741 cggagttgac taatgttgtg ggaaattgga gcgataagcg tgcttctgcc gtggccagga 1801 caacgtatac tcatcagata acagcaatac ctgatcacta cttcgcacta gtttctcggt 1861 actatgcata tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa 1921 ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc 1981 ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc tacataaata 2041 gacgcatatg gatccggccg atgggcaagg gcgaggaact gttcactggc gtggtcccaa 2101 tcctggtgga actggatggt gatgtgaacg ggcacaagtt ctccgtcagc ggagagggtg 2161 aaggtgatgc cacctacgga aagctcaccc tgaagttcat ctgcactacc ggaaagctcc 2221 ctgttccgtg gccaaccctc gtcaccactt tcacctacgg tgttcagtgc ttctcccggt 2281 acccagatca catgaagcag catgacttct tcaagagcgc catgcccgaa ggctacgtgc 2341 aagaaaggac tatcttcttc aaggatgacg ggaactacaa gacacgtgcc gaagtcaagt 2401 tcgaaggtga taccctggtg aaccgcatcg agctgaaagg taagtttctg cttctacctt 2461 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt 2521 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt 2581 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt atcgatttca 2641 aggaagatgg aaacatcctc ggacacaagc tggagtacaa ctacaactcc cacaacgtat 2701 acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc aggcacaaca 2761 tcgaagatgg aagcgtgcaa ctggcggacc actaccagca gaacacgccc atcggcgatg 2821 gccctgtcct gctgccggac aaccattacc tgtccacgca atctgccctc tccaaggacc 2881 ccaacgagaa gagggaccac atggtcctgc tggagttcgt gacggctgct gggatcacgc 2941 atggcatgga tgaactctac aagtgactcg acgatcgttc aaacatttgg caataaagtt 3001 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 3061 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 3121 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 3181 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcggtac ctataacttc 3241 gtataatgta tactatacga agttatttaa attggccccc atggcctcta gagagctcca 3301 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt 3361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 3421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 3481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 3541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 3601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 3661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 3721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 4021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 4081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 4141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 4201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 4261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 4321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 4381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 4441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 4501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 4561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 4621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 4681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 4741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 4861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 5041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 5101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 5161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 5221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 5281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 5341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 5401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 5461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg 5521 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 5581 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 5641 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 5701 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 5761 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 5821 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 5881 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 5941 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc 6001 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 6061 aagttgggta acgccagggt tttcccagtc acgacgt //