LOCUS       p35S-Gusi-NosK          5828 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  577p35S-Gusi-NosK rotated to position 5751
ACCESSION   p35S-Gusi-NosK
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pGusi-BM*
               Fragment ends: HindIII and NotI
               Fragment size: 5263
            Insert molecule:  35SNot-Hind*
               Fragment ends: NotI and HindIII
               Fragment size: 565
            Primer for gene synthesis
            M13RY	CACACAGGAAACAGCTATGAC
            p35SHind	CCCTCTaagcttgagccatAATTCCTGCAGCCC
            Sequencing primers	
            M13RY	CACACAGGAAACAGCTATGAC
            
FEATURES             Location/Qualifiers
     misc_feature    105..617
                     /gene="35S'"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             651..1071
                     /gene="GUS"
                     /product="GUS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="GUS"
                     /SECDescr="GUS"
     misc_feature    1072..1260
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Intron"
                     /SECDescr="Intron"
     CDS             1261..2687
                     /gene="GUS"
                     /product="GUS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="GUS"
                     /SECDescr="GUS"
     misc_feature    2748..3011
                     /gene="NOS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS"
                     /SECDescr="Terminator"
     misc_feature    3828..4688
                     /gene="Amp"
                     /product="Ampecillin resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="Ampecillin resistence"
     CDS             4731..5447
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagaataga ggacctaaca
      121 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
      181 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
      241 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
      301 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
      361 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
      421 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      481 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
      541 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      601 catttggaga gaacacgggg gactagtgga tcccccgggc tgcaggaatt atggctcaag
      661 cttacgcgtg tcgacgtcag tcccttatgt tacgtcctgt agaaacccca acccgtgaaa
      721 tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattggtc
      781 agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta
      841 acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg
      901 aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca
      961 ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata
     1021 cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtaagtttc
     1081 tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat ataatatttc
     1141 aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata
     1201 agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag
     1261 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
     1321 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     1381 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     1441 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
     1501 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
     1561 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
     1621 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     1681 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     1741 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     1801 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1861 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1921 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     1981 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     2041 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     2101 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     2161 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     2221 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     2281 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     2341 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     2401 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     2461 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     2521 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     2581 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     2641 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc aacaactctc
     2701 ctggcgcacc atcgtcggct acagcctcgg gaattgctac cgagctcgaa tttccccgat
     2761 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2821 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2881 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2941 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     3001 ttactagatc gctcgacagg ccttaagggc cagatcttgg gcccggtacc caattcgccc
     3061 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3121 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3241 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3301 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3361 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3421 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3481 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3541 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3601 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3661 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4981 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5701 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5761 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5821 aacaattt
//
