LOCUS       p35s-dmolgusI           4579 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of p35S-Gusi-Nos* into 986pUbiM-dmolgusI*
ACCESSION   p35s-dmolgusI
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  986pUbiM-dmolgusI*
               Fragment ends: SpeI and NotI
               Fragment size: 4050
            Insert molecule:  p35S-Gusi-Nos*
               Fragment ends: NotI and SpeI
               Fragment size: 529
FEATURES             Location/Qualifiers
     misc_feature    76..593
                     /gene="35S'"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             659..1273
                     /gene="ogus"
                     /product="plant codon optimized gus"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1274..1462
                     /gene="int"
                     /product="StLSI Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="StLSI Intron"
     CDS             1463..2659
                     /gene="ogus"
                     /product="plant codon optimized gus"
                     /codon_start=2
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2727..2980
                     /gene="NOS"
                     /product="NOS-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3062..3924
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    join(3925..4579,1..10)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actagaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
      121 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
      181 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
      241 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
      301 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      361 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      421 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      481 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      541 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactagttg
      601 gatcccccgg gctgcaggaa ttatggctca agcttacgcg tgtcgacgtc agtcccttat
      661 gcttagacct gttgagactc caacaaggga aatcaagaaa ctcgatggct tgtgggcttt
      721 ctctctggac agagagaact gcggaattgg tcagagatgg tgggaatccg cccttcaaga
      781 gtcaagagca atcgctgtgc cgggcagctt taatgaccag ttcgccgatg cagatattag
      841 gaactacgct ggaaatgtct ggtatcaaag agaagtgttt atccctaagg gttgggccgg
      901 acagaggatt gttctcagat tcgacgcagt cacccactac ggcaaagttt gggtgaacaa
      961 tcaagaagtc atggagcatc agggtggcta tactccattt gaagctgatg ttacacctta
     1021 cgtgatcgcc ggaaagtctg tcaggattac cgtttgtgtg aacaatgagt tgaactggca
     1081 aactatcccg ccaggcatgg ttattacaga tgaaaatggt aagaagaaac agtcctattt
     1141 ccacgacttc tttaactacg ctggaatcca tagatcagtg atgctgtata ccactccgaa
     1201 tacatgggtc gatgacatta ccgttgtgac tcacgtcgca caagattgca accatgccag
     1261 cgttgactgg caggtaagtt tctgcttcta cctttgatat atatataata attatcatta
     1321 attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc
     1381 aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg
     1441 accaaaattt gttgatgtgc aggtggtcgc taatggtgat gtttctgtgg agcttaggga
     1501 cgcagatcaa caagtcgttg ctacaggcca aggaacctcc ggtactctcc aagtggttaa
     1561 ccctcacttg tggcaaccag gcgaaggata cctgtatgag ctttgtgtga cagccaaatc
     1621 acagaccgag tgcgatatct acccgctcag agtcggtatt agatctgttg ctgtgaaggg
     1681 cgaacagttc cttatcaacc acaagccatt ttatttcact ggatttggta gacatgagga
     1741 cgcagatttg aggggcaaag gattcgacaa tgtcctgatg gttcacgatc atgccctcat
     1801 ggactggatt ggtgctaaca gctacagaac atctcactac ccttatgccg aagagatgtt
     1861 ggattgggca gatgaacacg gcatcgtggt cattgacgag accgctgccg ttggatttaa
     1921 tctttccctc ggtatcggct tcgaggcagg aaacaagcct aaagaactgt actcagagga
     1981 agctgtgaat ggtgagactc aacaggccca tcttcaagct attaaggagt tgatcgcaag
     2041 ggataagaac cacccgtctg ttgtgatgtg gagcattgct aatgaaccag acacaagacc
     2101 tcagggagcc agggagtatt tcgcaccgct cgctgaagcc accagaaaac ttgatccaac
     2161 taggcctatc acctgtgtca acgttatgtt ttgcgacgct catacagata ctatttccga
     2221 cctgttcgat gtgctctgtt tgaatagata ctatggctgg tacgtccaat caggcgatct
     2281 ggagacagca gaaaaagttc ttgagaaaga gttgctcgcc tggcaggaaa agctgcacca
     2341 accgatcatt atcaccgagt atggcgtgga cactcttgct ggactccatt ctatgtatac
     2401 agatatgtgg agcgaagagt accagtgcgc atggttggat atgtaccaca gggtctttga
     2461 cagagtttcc gccgtggtcg gtgaacaagt ttggaacttc gctgactttg caacctcaca
     2521 gggcattctg agggtgggtg gaaataagaa aggcatcttc actagagata ggaagccaaa
     2581 atctgccgct tttcttctcc aaaagagatg gacaggaatg aacttcggtg agaaacctca
     2641 gcaaggcgga aagcagtgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc
     2701 gggaattgct accgagctca gtcgacgatc gttcaaacat ttggcaataa agtttcttaa
     2761 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     2821 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     2881 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     2941 ataaattatc gcgcgcggtg tcatctatgt tactagatcg gtaccattta aattggcccc
     3001 caaggcctga tcttgagagc tattctagtc actcatataa tgtctgcata ggaggtcagc
     3061 catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3121 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3181 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3241 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3301 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3361 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3421 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3481 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3541 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3601 gccagcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3661 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3721 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3781 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3841 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3901 ctcactgatt aagcattggt gatctcatgg ccaaaatccc ttaacgtgag ttttcgttcc
     3961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4141 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4561 gctcgtcagg ggggcggag
//
