LOCUS       pD35-ocs                3957 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of D35-Eag-Bam* into pOCS-BM*
ACCESSION   pD35-ocs
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pOCS-BM*
               Fragment ends: BamHI and NotI
               Fragment size: 3145
            Insert molecule:  D35-Eag-Bam*
               Fragment ends: EagI and BamHI
               Fragment size: 812
            Primer for gene synthesis
            D35Eag	CCCTTcggccgCTAGATCGGGCCAA
            D35Bam	CCCTggatccTactagtCGAGAGAGATAGATTTGTAGA
            Sequencing primers	
            M13RY	CACACAGGAAACAGCTATGAC
            OR	CATGCGATCATAGGCGTCTC
FEATURES             Location/Qualifiers
     misc_feature    110..894
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     822..828
                     /label=TATA
                     /SECDrawAs="Label"
     misc_feature    946..1141
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    1957..2817
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             2860..3576
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgct agatcgggcc aacatggtgg
      121 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
      181 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
      241 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
      301 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
      361 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      421 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      481 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      541 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      601 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      661 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      721 gaccttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
      781 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      841 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgact
      901 agtaggatcc cccgggctgc aggaattcaa gcttacgcgt gtcgaccctg ctttaatgag
      961 atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa
     1021 aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata
     1081 tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt actgattgtc
     1141 ctcgacaggc cttaagggcc agatcttggg cccggtaccc aattcgccct atagtgagtc
     1201 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1261 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1321 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     1381 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1441 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1621 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1801 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1861 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1981 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2041 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2101 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2161 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2221 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2281 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2341 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2401 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2461 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2521 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2581 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2641 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2701 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2761 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2821 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2881 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2941 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3001 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3061 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3121 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3601 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3661 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3721 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3781 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3841 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3901 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaattt
//
