LOCUS       pENTK                   2605 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  ENTK-Eco* Circularized
ACCESSION   pENTK
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Product created by PCR amplification and EcoRI digest
            Molecule 253pEntr-DCS
            Forward primer (EMCSF) bp pos 731
            Reverse primer (EMCSR) bp pos 669
            Primer for gene synthesis
            EMCSF	CCCgaattctaagcttgacgcgttagtcgaCCGACCCAGCTTTCT
            EMCSR	CCCgaattcactgcagacccgggtggatcctactagtCGGAGCCTGCTTTTTTGT
            Sequencing primers	After EcoRI digest and column purification
            EntF	GTTCACCGACAAACAACAGA
            EntR	CCCGTTGAATATGGCTCATAA
FEATURES             Location/Qualifiers
     misc_feature    567..666
                     /gene="attL1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     727..729
                     /label=frame
                     /SECDrawAs="Label"
     misc_feature    728..827
                     /gene="attL2"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             997..1806
                     /gene="Kan"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1906..2541
                     /gene="ori pBR322"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
       61 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      181 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
      241 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      301 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      361 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      421 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      481 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      541 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
      601 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
      661 caggctccga ctagtaggat ccacccgggt ctgcagtgaa ttctaagctt gacgcgttag
      721 tcgaccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      781 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
      841 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
      901 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      961 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1021 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1081 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1141 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1201 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1261 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1321 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1381 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1441 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1501 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1561 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1621 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1681 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1741 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     1801 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     1861 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     1921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2341 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2581 tgctggcctt ttgctcacat gttct
//
