LOCUS pEntA 2301 bp DNA circular SYN 20-JUN-2013 DEFINITION 473pEntA rotated to position 2204 ACCESSION pEntA KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 2301) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: LC-Nco-Nhe* Fragment ends: NheI and NcoI Fragment size: 1852 Insert molecule: 253pEntr-DCS* Fragment ends: NheI and BspHI Fragment size: 449 GELR Primer for gene synthesis PBR-NcoR CCCTTccatggCCTCGTGATACGCCTATT PBR-Nhe CCTTgctagcGGCCAGGAACCGTAAA Sequencing primers PBRR CTCTGACTTGAGCGTCGATTT PBRF GCCACCTGACGTCTAAGAAA FEATURES Location/Qualifiers misc_feature 158..257 /gene="attL1" /SECDrawAs="Region" /SECStyleId=1 misc_signal 324..326 /label=frame /SECDrawAs="Label" misc_feature 325..424 /gene="attL2" /SECDrawAs="Region" /SECStyleId=1 misc_feature 698..704 /gene="P3" /product="P3" /SECDrawAs="Region" /SECStyleId=1 CDS 739..1599 /gene="amp" /product="beta-lactamase, ampicillin resistance" /SECDrawAs="Gene" /SECStyleId=1 misc_feature complement(join(1647..2301,1..57)) /gene="ColE1" /product="Ori" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 61 gccagcaacg cggccttttt acggttcctg gccgctagca tggatgtttt cccagtcacg 121 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact 181 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt 241 gtacaaaaaa gcaggctccg cggccgctaa ggccggccag aattctggta cctgtcgacg 301 ggatcctact agtagggcgc gccgacccag ctttcttgta caaagttggc attataagaa 361 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 421 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 481 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 541 catcatggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 961 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac 1021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 1081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 1141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 1201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 1261 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc 1321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 1381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 1441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 1501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 1561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 1621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 1681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2281 agtcctgtcg ggtttcgcca c //