LOCUS       pUbi-BA                 3522 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of Ubi-Nos-BsiW-Sph* into 508pD2LC1*
ACCESSION   pUbi-BA
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  508pD2LC1*
               Fragment ends: SphI and BsiWI
               Fragment size: 1684
            Insert molecule:  Ubi-Nos-BsiW-Sph*
               Fragment ends: BsiWI and SphI
               Fragment size: 1838
            Primer for gene synthesis
            UbiBsiW	CCCTcgtacgTGCAGTGCAGCGTGA
            NosSph	CCCTTgcatGCGTCGAGCGATCTA
            Sequencing primers	
            F	CCTATGGAAAAACGCCAGC
            RN	GGCTGACCTCCTATGCAGAT
            Sequencing primers	
            UF1	CGAGTAGATAATGCCAGCC
            
            
            
FEATURES             Location/Qualifiers
     misc_feature    85..1592
                     /gene="'Ubi-int"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1071..1590
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1653..1906
                     /gene="NOS-T"
                     /product="NOS-T"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(1995..2659)
                     /gene="ColE1''"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2437
                     /gene="LC"
                     /product="Low copy origin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="LC"
                     /SECDescr="Low copy origin"
     CDS             complement(2660..3522)
                     /gene="Amp''"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggctgacctc ctatgcagat atcttatgag tgactagaat agctctcaag atcaggcctt
       61 gggggccaat ttaaatggcg tacgtgcagt gcagcgtgac ccggtcgtgc ccctctctag
      121 agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac
      181 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa
      241 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta
      301 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt
      361 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca
      421 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta
      481 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt
      541 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca
      601 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat
      661 gccagcctgt taaacgccgt cgatcgacga gtctaacgga caccaaccag cgaaccagca
      721 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
      781 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
      841 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
      901 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      961 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
     1021 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1081 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1141 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1201 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1261 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1321 ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc
     1381 agcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt
     1441 tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat
     1501 ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc
     1561 tcaccctgtt gtttggtgtt acttctgcag gtactagtgg atcccccggg ctgcaggaat
     1621 tcaagcttac gcgtgtcgac tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     1681 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     1741 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     1801 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     1861 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgctcg acgcatgctg
     1921 gtacctgacg tctgcggccg cattaattaa ggccctaagg gcctgatctg tataaaggcc
     1981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2221 taggtcgttc gctccaagct gggctgtgag cacgaacccc ccgttcagcc cgaccgctgc
     2281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2401 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2641 taagggattt tggccatgag atcaccaatg cttaatcagt gaggcaccta tctcagcgat
     2701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2941 gccagttaat agtttgcgca acgttgttgc cattgctgct ggcatcgtgg tgtcacgctc
     3001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3181 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3241 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     3301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgggcaccca actgatcttc
     3421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
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