LOCUS       pUbiAT-OCS              4634 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of pUbiAT-Gus-Or* into pOCS-BM*
ACCESSION   pUbiAT-OCS
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pOCS-BM*
               Fragment ends: SpeI and EagI
               Fragment size: 3151
            Insert molecule:  pUbiAT-Gus-Or*
               Fragment ends: EagI and SpeI
               Fragment size: 1483
FEATURES             Location/Qualifiers
     misc_feature    26..1503
                     /gene="UbiAT-Pro"
                     /product="Promotor Ubi A.thaliana"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     1099..1102
                     /label=TATA
                     /SECDrawAs="Label"
     CDS             1132..1195
                     /gene="Ubi cds 5`"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1198..1501
                     /gene="intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     1263..1266
                     /label=TATA
                     /SECDrawAs="Label"
     misc_feature    1551..1746
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    2562..3422
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    3465..4181
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gagctctcta gaggccatgg cggccgcttt ccttacattc tgagcctctt tccttctaat
       61 ccactcatct gcatcttctt gtgtccttac taatacctca ttggttccaa attccctccc
      121 tttaagcacc agctcgtttc tgttcttcca cagcctccca agtatccaag ggactaaagc
      181 ctccacattc ttcagatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt
      241 atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa
      301 tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga
      361 ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta
      421 aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc
      481 aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa
      541 cactaaaaaa ttaaaagaaa tggataattt cacaatatgt tatacgataa agaagttact
      601 tttccaagaa attcactgat tttataagcc cacttgcatt agataaatgg caaaaaaaaa
      661 caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac gcttcaatgc
      721 agtgggaccc acggttcaat tattgccaat tttcagctcc accgtatatt taaaaaataa
      781 aacgataatg ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg gctggatctt
      841 atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg tcaaagtggt
      901 tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc
      961 taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt
     1021 cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt
     1081 cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag
     1141 atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta
     1201 aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt
     1261 cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt
     1321 gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc
     1381 aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg
     1441 tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca
     1501 gatactagtg gatcccccgg gctgcaggaa ttcaagctta cgcgtgtcga ccctgcttta
     1561 atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt
     1621 tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat
     1681 gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga
     1741 ttgtcctcga caggccttaa gggccagatc ttgggcccgg tacccaattc gccctatagt
     1801 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg
     1981 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2041 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2101 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2161 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2221 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2281 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2341 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2401 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     2461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3721 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4381 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4441 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     4501 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     4561 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
     4621 gggaacaaaa gctg
//
