LOCUS       pUbiAtmu-ocs            4634 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of pUbiAT-Gus-Mu* into pOCS-BM*
ACCESSION   pUbiAtmu-ocs
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pOCS-BM*
               Fragment ends: SpeI and NotI
               Fragment size: 3151
            Insert molecule:  pUbiAT-Gus-Mu*
               Fragment ends: NotI and SpeI
               Fragment size: 1483
            Sequencing primers	
            OR	CATGCGATCATAGGCGTCTC
FEATURES             Location/Qualifiers
     misc_feature    126..1601
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1230..1293
                     /gene="Ubi cds 5`"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1296..1599
                     /gene="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1649..1844
                     /gene="ocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ocs"
                     /SECDescr="Terminator"
     misc_feature    2660..3520
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    3563..4279
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgctttcc ttacattctg agcctctttc cttctaatcc actcatctgc atcttcttgt
      181 gtccttacta atacctcatt ggttccaaat tccctccctt taagcaccag ctcgtttctg
      241 ttcttccaca gcctcccaag tatccaaggg actaaagcct ccacattctt cagatcagga
      301 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg
      361 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt
      421 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg
      481 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc
      541 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat
      601 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg
      661 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
      721 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
      781 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
      841 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
      901 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg
      961 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg
     1021 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa
     1081 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct
     1141 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata
     1201 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta
     1261 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc
     1321 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga
     1381 ttctgttaat cttagatcga agacgatttt ctgggtttga tcgttagata tcatcttaat
     1441 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
     1501 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
     1561 tcgaatttgt cgattaatct gagtttttct gattaacagg tactagtgga tcccccgggc
     1621 tgcaggaatt caagcttacg cgtgtcgacc ctgctttaat gagatatgcg agacgcctat
     1681 gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt
     1741 agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc
     1801 gtatttttat gaataatatt ctccgttcaa tttactgatt gtcctcgaca ggccttaagg
     1861 gccagatctt gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg
     1921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1981 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2041 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa
     2101 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     2161 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     2221 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     2281 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     2341 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     2401 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     2461 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     2521 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2581 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2641 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2701 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2761 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2821 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2881 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2941 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3001 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3061 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3121 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3181 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3241 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3301 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3361 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3421 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3481 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3541 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3601 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3661 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3901 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3961 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4021 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4081 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4141 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4201 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4261 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4321 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4381 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4441 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4501 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4561 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4621 ggctcgtatg ttgt
//
