LOCUS       pUbiM-dmoLgus           5383 bp    DNA     circular SYN 20-JUN-2013
DEFINITION  Ligation of 955pUbiM-dmoLgusxx* into 955pUbiM-dmoLgusx*
ACCESSION   pUbiM-dmoLgus
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  955pUbiM-dmoLgusx* Woche 32 Klon1
               Fragment ends: SacI and EcoRV
               Fragment size: 4301
            Insert molecule:  955pUbiM-dmoLgusxx* Woche 32 Klon3
               Fragment ends: EcoRV and SacI
               Fragment size: 1082
            Sequencing Primer:
            UF2
            dmog
            NosR
FEATURES             Location/Qualifiers
     misc_feature    77..1583
                     /gene="UbiZm"
                     /product="Promotor Ubi Zea mays"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1064..1583
                     /gene="Intron"
                     /product="Intron UbiZm"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1652..3463
                     /gene="ogus"
                     /product="plant codon optimized gus "
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="ogus"
                     /SECDescr="plant codon optimized gus "
     misc_feature    3531..3784
                     /gene="NOS"
                     /product="NOS-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3866..4728
                     /gene="Amp"
                     /product="Amp-DCS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    join(4729..5383,1..10)
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cctatggaaa aacgccagca agcggccttt atacagatca ggcccttagg gccttaatta
       61 atgcggccgc actaggctgc agtgcagcgt gacccggtcg tgcccctctc tagagataat
      121 gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg
      181 aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa taatataatc
      241 tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg
      301 tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc
      361 atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt
      421 agtacatcca tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc
      481 tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta
      541 tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc
      601 tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc
      661 tgttaaacgc cgtcgatcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc
      721 gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga
      781 gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag
      841 cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta
      901 cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag
      961 acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa
     1021 ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc
     1081 cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt
     1141 agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag
     1201 cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt
     1261 ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc gatctaggat
     1321 aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct
     1381 attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt
     1441 attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta
     1501 gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct
     1561 gttgtttggt gttacttctg caggtactag ttggatcccc cgggctgcag gaattatggc
     1621 tcaagcttac gcgtgtcgac gtcagtccct tatgcttaga cctgttgaga ctccaacaag
     1681 ggaaatcaag aaactcgatg gcttgtgggc tttctctctg gacagagaga actgcggaat
     1741 tggtcagaga tggtgggaat ccgcccttca agagtcaaga gcaatcgctg tgccgggcag
     1801 ctttaatgac cagttcgccg atgcagatat taggaactac gctggaaatg tctggtatca
     1861 aagagaagtg tttatcccta agggttgggc cggacagagg attgttctca gattcgacgc
     1921 agtcacccac tacggcaaag tttgggtgaa caatcaagaa gtcatggagc atcagggtgg
     1981 ctatactcca tttgaagctg atgttacacc ttacgtgatc gccggaaagt ctgtcaggat
     2041 taccgtttgt gtgaacaatg agttgaactg gcaaactatc ccgccaggca tggttattac
     2101 agatgaaaat ggtaagaaga aacagtccta tttccacgac ttctttaact acgctggaat
     2161 ccatagatca gtgatgctgt ataccactcc gaatacatgg gtcgatgaca ttaccgttgt
     2221 gactcacgtc gcacaagatt gcaaccatgc cagcgttgac tggcaggtgg tcgctaatgg
     2281 tgatgtttct gtggagctta gggacgcaga tcaacaagtc gttgctacag gccaaggaac
     2341 ctccggtact ctccaagtgg ttaaccctca cttgtggcaa ccaggcgaag gatacctgta
     2401 tgagctttgt gtgacagcca aatcacagac cgagtgcgat atctacccgc tcagagtcgg
     2461 tattagatct gttgctgtga agggcgaaca gttccttatc aaccacaagc cattttattt
     2521 cactggattt ggtagacatg aggacgcaga tttgaggggc aaaggattcg acaatgtcct
     2581 gatggttcac gatcatgccc tcatggactg gattggtgct aacagctaca gaacatctca
     2641 ctacccttat gccgaagaga tgttggattg ggcagatgaa cacggcatcg tggtcattga
     2701 cgagaccgct gccgttggat ttaatctttc cctcggtatc ggcttcgagg caggaaacaa
     2761 gcctaaagaa ctgtactcag aggaagctgt gaatggtgag actcaacagg cccatcttca
     2821 agctattaag gagttgatcg caagggataa gaaccacccg tctgttgtga tgtggagcat
     2881 tgctaatgaa ccagacacaa gacctcaggg agccagggag tatttcgcac cgctcgctga
     2941 agccaccaga aaacttgatc caactaggcc tatcacctgt gtcaacgtta tgttttgcga
     3001 cgctcataca gatactattt ccgacctgtt cgatgtgctc tgtttgaata gatactatgg
     3061 ctggtacgtc caatcaggcg atctggagac agcagaaaaa gttcttgaga aagagttgct
     3121 cgcctggcag gaaaagctgc accaaccgat cattatcacc gagtatggcg tggacactct
     3181 tgctggactc cattctatgt atacagatat gtggagcgaa gagtaccagt gcgcatggtt
     3241 ggatatgtac cacagggtct ttgacagagt ttccgccgtg gtcggtgaac aagtttggaa
     3301 cttcgctgac tttgcaacct cacagggcat tctgagggtg ggtggaaata agaaaggcat
     3361 cttcactaga gataggaagc caaaatctgc cgcttttctt ctccaaaaga gatggacagg
     3421 aatgaacttc ggtgagaaac ctcagcaagg cggaaagcag tgaatcaaca actctcctgg
     3481 cgcaccatcg tcggctacag cctcgggaat tgctaccgag ctcagtcgac gatcgttcaa
     3541 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     3601 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     3661 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     3721 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     3781 atcggtacca tttaaattgg cccccaaggc ctgatcttga gagctattct agtcactcat
     3841 ataatgtctg cataggaggt cagccatgag tattcaacat ttccgtgtcg cccttattcc
     3901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4081 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4381 aaacgacgag cgtgacacca cgatgccagc agcaatggca acaacgttgc gcaaactatt
     4441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtgatctc atggccaaaa
     4741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gag
//
