LOCUS       p1U-SG                  4255 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  Ligation of Lox-Ubi-SG* into pD1AF*
ACCESSION   p1U-SG
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    78..1586
                     /gene="Ubi-int"
                     /product="Promotor"
     misc_feature    1065..1584
                     /gene="Intron"
                     /product="Intron"
     misc_feature    1597..2369
                     /gene="SG"
                     /product="GUS"
     misc_feature    2403..2656
                     /gene="NOS"
                     /product="NOS-Terminator"
     CDS             2738..3600
                     /gene="Amp"
                     /product="Amp-DCS1"
     misc_feature    3601..10
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 cctatggaaa aacgccagca acgcggcctt tatacagatc aggcccttag ggccttaatt
       61 aatgcggccg cactaggctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa
      121 tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt
      181 gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga ataatataat
      241 ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg
      301 gtctaaagga caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg
      361 catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat
      421 tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat
      481 ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt
      541 atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc
      601 ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc
      661 ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg
      721 cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg
      781 agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga
      841 gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct
      901 acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata
      961 gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca
     1021 accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct
     1081 cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt tagggcccgg
     1141 tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta
     1201 gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg
     1261 tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat cgatctagga
     1321 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1381 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1441 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1501 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1561 tgttgtttgg tgttacttct gcaggtacta gttggatcct gtagaaaccc caacccgtga
     1621 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
     1681 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
     1741 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
     1801 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
     1861 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
     1921 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
     1981 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
     2041 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
     2101 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
     2161 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
     2221 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
     2281 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
     2341 actgtgcgtc acagccaaaa gccagacaga attccaagct tggcgcgccg agctcagtcg
     2401 acgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     2461 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     2521 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     2581 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     2641 ctatgttact agatcggtac catttaaatt ggcccccaag gcctgatctt gagagctatt
     2701 ctagtcactc atataatgtc tgcataggag gtcagccatg agtattcaac atttccgtgt
     2761 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2821 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2881 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2941 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     3001 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3061 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3121 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3181 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3241 tgaagccata ccaaacgacg agcgtgacac cacgatgcca gcagcaatgg caacaacgtt
     3301 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3361 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3421 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3481 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3541 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtgatc
     3601 tcatggccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3661 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3721 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3781 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3841 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3901 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3961 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4021 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4081 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4141 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4201 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggag
//
