LOCUS       p35-iF2-1               4731 bp    DNA     circular SYN 13-JUL-2010
DEFINITION  35-intron-fad2-BM rotated to position 4704
ACCESSION   p35-iF2-1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    34..452
                     /gene="35S-Pro"
                     /product="Promotor-Region"
     CDS             423..429
                     /gene="(null)"
                     /product="TATA-box"
     misc_feature    486..494
                     /gene="(null)"
                     /product="splicing-site1"
     misc_feature    489..1620
                     /gene="Intron fad2"
                     /product="Intron Fad2"
     misc_feature    1618..1622
                     /gene="(null)"
                     /product="splicing-site2"
     misc_feature    1648..1843
                     /gene="OCS"
                     /product="Terminator"
     CDS             2659..3519
                     /gene="Amp"
                     /product="ORF-1"
     misc_feature    3562..4278
                     /gene="ColE1"
                     /product="ColE1"
ORIGIN      
        1 gagctctcta gaggccatgg cggccgcact agatcaacat ggtggagcac gacactctcg
       61 tctactccaa gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc
      121 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
      181 tcgaaaggac agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa
      241 aggctatcgt tcaagaatgc ctctaccgac agtggtccca aagatggacc cccacccacg
      301 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
      361 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
      421 tctatataag gaagttcatt tcatttggag aggacactag ttggatcccc cgggctgcag
      481 gaattcaggt ccgtcgcttc tcttccattt cttctcattt tcgattttga ttcttatttc
      541 tttccagtag ctcctgctct gtgaatttct ccgctcacga tagatctgct tatactcctt
      601 acattcaacc ttagatctgg tctcgattct ctgtttctct gtttttttct tttggtcgag
      661 aatctgatgt ttgtttatgt tctgtcacca ttaataataa tgaactctct cattcataca
      721 atgattagtt tctctcgtct acaaaacgat atgttgcatt ttcacttttc ttcttttttt
      781 ctaagatgat ttgctttgac caatttgttt agatctttat tttattttat tttctggtgg
      841 gttggtggaa attgaaaaaa aaaaaaaaca gcataaattg ttatttgtta atgtattcat
      901 tttttggcta tttgttctgg gtaaaaatct gcttctacta ttgaatcttt cctggatttt
      961 ttactcctat tgggttttta tagtaaaaat acataataaa aggaaaacaa aagttttata
     1021 gattctctta aaccccttac gataaaagtt ggaatcaaaa taattcagga tcagatgctc
     1081 tttgattgat tcagatgcga ttacagttgc atggcaaatt ttctagatcc gtcgtcacat
     1141 tttattttct gtttaaatat ctaaatctga tatatgatgt cgacaaattc tggtggctta
     1201 tacatcactt caactgtttt cttttggctt tgtttgtcaa cttggttttc aatacgattt
     1261 gtgatttcga tcgctgaatt tttaatacaa gcaaactgat gttaaccaca agcaagagat
     1321 gtgacctgcc ttattaacat cgtattactt actactagtc gtattctcaa cgcaatcgtt
     1381 tttgtatttc tcacattatg ccgcttctct actctttatt ccttttggtc cacgcatttt
     1441 ctatttgtgg caatcccttt cacaacctga tttcccactt tggatcattt gtctgaagac
     1501 tctcttgaat cgttaccact tgtttcttgt gcatgctctg ttttttagaa ttaatgataa
     1561 aactattcca tagtcttgag ttttcagctt gttgattctt ttgcttttgg ttttctgcag
     1621 gtcaattggt gtgatcacgc gtgtcgaccc tgctttaatg agatatgcga gacgcctatg
     1681 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
     1741 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
     1801 tatttttatg aataatattc tccgttcaat ttactgattg tcctcgacag gccttaaggg
     1861 ccagatcttg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca attcactggc
     1921 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1981 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2041 ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa
     2101 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2161 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2221 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2281 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2341 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2401 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2461 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2521 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2581 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2641 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2701 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2761 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2821 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2881 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2941 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3001 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3061 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3181 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3481 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3541 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3601 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3901 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3961 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4021 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4081 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4141 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4201 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4261 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4321 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4381 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4441 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4501 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4561 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4621 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4681 catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct g
//
